2YJK

Structure of Dps from MICROBACTERIUM ARBORESCENS in the high iron form

2YJK の概要

• エントリーDOI: 10.2210/pdb2yjk/pdb
• 関連するPDBエントリー: 2YJJ
• 分子名称: AFP, Iron(II) oxide, FE (III) ION, ... (4 entities in total)
• 機能のキーワード: metal-binding protein, iron uptake, ferritin fold, metal binding protein
• 由来する生物種: MICROBACTERIUM ARBORESCENS
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 208538.54

構造登録者

Zeth, K.,Buecheler, R.,Boland, W. (登録日: 2011-05-19, 公開日: 2011-06-01, 最終更新日: 2023-12-20)

主引用文献

Pesek, J.,Buecheler, R.,Albrecht, R.,Boland, W.,Zeth, K.
Structure and Mechanism of Iron Translocation by a Dps Protein from Microbacterium Arborescens.
J.Biol.Chem., 286:34872-, 2011

PubMed Abstract: Dps (DNA protection during starvation) enzymes are a major class of dodecameric proteins that bacteria use to detoxify their cytosol through the uptake of reactive iron species. In the stationary growth phase of bacteria, Dps enzymes are primarily used to protect DNA by biocrystallization. To characterize the wild type Dps protein from Microbacterium arborescens that displays additional catalytic functions (amide hydrolysis and synthesis), we determined the crystal structure to a resolution of 2.05 Å at low iron content. The structure shows a single iron at the ferroxidase center coordinated by an oxo atom, one water molecule, and three ligating residues. An iron-enriched protein structure was obtained at 2 Å and shows the stepwise uptake of two hexahydrated iron atoms moving along channels at the 3-fold axis before a restriction site inside the channels requires removal of the hydration sphere. Supporting biochemical data provide insight into the regulation of this acylamino acid hydrolase. Moreover, the peroxidase activity of the protein was determined. The influence of iron and siderophores on the expression of acylamino acid hydrolase was monitored during several stages of cell growth. Altogether our data provide an interesting view of an unusual Dps-like enzyme evolutionarily located apart from the large Dps sequence clusters.

PubMed: 21768097
DOI: 10.1074/JBC.M111.246108

実験手法

X-RAY DIFFRACTION (2 Å)