2YI8

Structure of the RNA polymerase VP1 from Infectious Pancreatic Necrosis Virus

2YI8 の概要

• エントリーDOI: 10.2210/pdb2yi8/pdb
• 関連するPDBエントリー: 2YI9 2YIA 2YIB
• 分子名称: RNA-DIRECTED RNA POLYMERASE, POTASSIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: transferase, reverse transcriptase, ipnv
• 由来する生物種: INFECTIOUS PANCREATIC NECROSIS VIRUS
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 448291.33

構造登録者

Graham, S.C.,Sarin, L.P.,Bahar, M.W.,Myers, R.A.,Stuart, D.I.,Bamford, D.H.,Grimes, J.M. (登録日: 2011-05-11, 公開日: 2011-07-20, 最終更新日: 2023-12-20)

主引用文献

Graham, S.C.,Sarin, L.P.,Bahar, M.W.,Myers, R.A.,Stuart, D.I.,Bamford, D.H.,Grimes, J.M.
The N-Terminus of the RNA Polymerase from Infectious Pancreatic Necrosis Virus is the Determinant of Genome Attachment.
Plos Pathog., 7:2085-, 2011

PubMed Abstract: The RNA-dependent RNA polymerase VP1 of infectious pancreatic necrosis virus (IPNV) is a single polypeptide responsible for both viral RNA transcription and genome replication. Sequence analysis identifies IPNV VP1 as having an unusual active site topology. We have purified, crystallized and solved the structure of IPNV VP1 to 2.3 Å resolution in its apo form and at 2.2 Å resolution bound to the catalytically-activating metal magnesium. We find that recombinantly-expressed VP1 is highly active for RNA transcription and replication, yielding both free and polymerase-attached RNA products. IPNV VP1 also possesses terminal (deoxy)nucleotide transferase, RNA-dependent DNA polymerase (reverse transcriptase) and template-independent self-guanylylation activity. The N-terminus of VP1 interacts with the active-site cleft and we show that the N-terminal serine residue is required for formation of covalent RNA:polymerase complexes, providing a mechanism for the genesis of viral genome:polymerase complexes observed in vivo.

PubMed: 21731487
DOI: 10.1371/JOURNAL.PPAT.1002085

実験手法

X-RAY DIFFRACTION (2.3 Å)