2YBB

Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876)

2YBB の概要

• エントリーDOI: 10.2210/pdb2ybb/pdb
• EMDBエントリー: 1876
• 分子名称: NADH-QUINONE OXIDOREDUCTASE SUBUNIT 1, CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL, CYTOCHROME B, ... (52 entities in total)
• 機能のキーワード: supercomplex b, mitochondria, respiratory chain, oxidoreductase, amphipol a8-35, random conical tilt
• 由来する生物種: THERMUS THERMOPHILUS; 詳細
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 1125224.33

構造登録者

Althoff, T.,Mills, D.J.,Popot, J.-L.,Kuehlbrandt, W. (登録日: 2011-03-02, 公開日: 2011-10-19, 最終更新日: 2024-11-20)

主引用文献

Althoff, T.,Mills, D.J.,Popot, J.-L.,Kuehlbrandt, W.
Arrangement of Electron Transport Chain Components in Bovine Mitochondrial Supercomplex I(1)III(2)Iv(1).
Embo J., 30:4652-, 2011

PubMed Abstract: The respiratory chain in the inner mitochondrial membrane contains three large multi-enzyme complexes that together establish the proton gradient for ATP synthesis, and assemble into a supercomplex. A 19-Å 3D map of the 1.7-MDa amphipol-solubilized supercomplex I(1)III(2)IV(1) from bovine heart obtained by single-particle electron cryo-microscopy reveals an amphipol belt replacing the membrane lipid bilayer. A precise fit of the X-ray structures of complex I, the complex III dimer, and monomeric complex IV indicates distances of 13 nm between the ubiquinol-binding sites of complexes I and III, and of 10-11 nm between the cytochrome c binding sites of complexes III and IV. The arrangement of respiratory chain complexes suggests two possible pathways for efficient electron transfer through the supercomplex, of which the shorter branch through the complex III monomer proximal to complex I may be preferred.

PubMed: 21909073
DOI: 10.1038/EMBOJ.2011.324

実験手法

ELECTRON MICROSCOPY (19 Å)