2Y8E

Crystal structure of D. melanogaster Rab6 GTPase bound to GMPPNP

2Y8E の概要

• エントリーDOI: 10.2210/pdb2y8e/pdb
• 分子名称: RAB-PROTEIN 6, SULFATE ION, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: hydrolase, nucleotide binding, gtp binding
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42067.26

構造登録者

Walden, M.,Edwards, T.A. (登録日: 2011-02-04, 公開日: 2011-07-06, 最終更新日: 2023-12-20)

主引用文献

Walden, M.,Jenkins, H.T.,Edwards, T.A.
Structure of the Drosophila Melanogaster Rab6 Gtpase at 1.4 A Resolution
Acta Crystallogr.,Sect.F, 67:744-, 2011

PubMed Abstract: Rab6 is a small GTPase that belongs to the p21 Ras superfamily. It is involved in vesicle trafficking between the Golgi apparatus and endosomes/ER in eukaryotes. The GDP-bound inactive protein undergoes conformational changes when the nucleotide is exchanged to GTP, allowing Rab6 to interact with a variety of different effector proteins. To further understand how these changes affect downstream protein binding, the crystal structure of Rab6 from Drosophila melanogaster has been solved to 1.4 Å resolution, the highest resolution for a Rab6 structure to date. The crystals belonged to space group C2, with unit-cell parameters a=116.5, b=42.71, c=86.86 Å, α=90, β=133.12, γ=90°. The model was refined to an R factor of 14.5% and an Rfree of 17.3%.

PubMed: 21795785
DOI: 10.1107/S1744309111017453

実験手法

X-RAY DIFFRACTION (1.39 Å)