2Y8C

Plasmodium falciparum dUTPase in complex with a trityl ligand

Summary for 2Y8C

• Entry DOI: 10.2210/pdb2y8c/pdb
• Related: 1VYQ
• Descriptor: DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE, (2S)-2-[(2,4-dioxopyrimidin-1-yl)methyl]-N-(2-hydroxyethyl)-4-trityloxy-butanamide, SULFATE ION, ... (4 entities in total)
• Functional Keywords: hydrolase, malaria
• Biological source: PLASMODIUM FALCIPARUM
• Total number of polymer chains: 3
• Total formula weight: 60542.40

Authors

Baragana, B.,McCarthy, O.,Sanchez, P.,Bosch, C.,Kaiser, M.,Brun, R.,Whittingham, J.L.,Roberts, S.,Zhou, X.-X.,Wilson, K.S.,Johansson, N.G.,Gonzalez-Pacanowska, D.,Gilbert, I.H. (deposition date: 2011-02-04, release date: 2012-03-07, Last modification date: 2023-12-20)

Primary citation

Baragana, B.,Mccarthy, O.,Sanchez, P.,Bosch, C.,Kaiser, M.,Brun, R.,Whittingham, J.L.,Roberts, S.,Zhou, X.-X.,Wilson, K.S.,Johansson, N.G.,Gonzalez-Pacanowska, D.,Gilbert, I.H.
Beta-Branched Acyclic Nucleoside Analogues as Inhibitors of Plasmodium Falciparum Dutpase
Bioorg.Med.Chem., 19:2378-, 2011

PubMed Abstract: We report a series of β-branched acyclic tritylated deoxyuridine analogues as inhibitors of Plasmodium falciparum deoxyuridine-5'-triphosphate nucleotidohydrolase (PfdUTPase), an enzyme involved in nucleotide metabolism that acts as first line of defence against uracil incorporation into DNA. Compounds were assayed against both PfdUTPase and intact parasites showing a correlation between enzyme inhibition and cellular assays. β-Branched acyclic uridine analogues described here showed equal or slightly better potency and selectivity compared with previously reported analogues. The best inhibitor gave a K(i) of 0.5 μM against PfdUTPase with selectivity greater than 200-fold compared to the corresponding human enzyme and sub-micromolar growth inhibition of P. falciparum (EC(50) 0.6 μM). A crystal structure of the complex of PfdUTPase with one of the inhibitors shows that this acyclic derivative binds to the active site in a similar manner to that previously reported for a tritylated cyclic deoxyuridine derivative.

PubMed: 21411327
DOI: 10.1016/J.BMC.2011.02.012

Experimental method

X-RAY DIFFRACTION (2.1 Å)