2Y7K

DntR Inducer Binding Domain in Complex with Salicylate. Monoclinic crystal form

2Y7K の概要

• エントリーDOI: 10.2210/pdb2y7k/pdb
• 関連するPDBエントリー: 1UTB 1UTH 2Y7P 2Y7R 2Y7W 2Y84
• 分子名称: LYSR-TYPE REGULATORY PROTEIN, 2-HYDROXYBENZOIC ACID (3 entities in total)
• 機能のキーワード: transcription, transcription factor, lysr type, transcription regulator
• 由来する生物種: BURKHOLDERIA SP. DNT
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 100236.13

構造登録者

Devesse, L.,Smirnova, I.,Lonneborg, R.,Kapp, U.,Brzezinski, P.,Leonard, G.A.,Dian, C. (登録日: 2011-01-31, 公開日: 2011-07-20, 最終更新日: 2024-05-08)

主引用文献

Devesse, L.,Smirnova, I.,Lonneborg, R.,Kapp, U.,Brzezinski, P.,Leonard, G.A.,Dian, C.
Crystal Structures of Dntr Inducer Binding Domains in Complex with Salicylate Offer Insights Into the Activation of Lysr-Type Transcriptional Regulators.
Mol.Microbiol., 81:354-, 2011

PubMed Abstract: Activation of LysR-type transcription factors (LTTRs) is thought to result from conformational changes that occur when inducer molecules bind to their Inducer Binding Domains (IBDs). However, the exact nature of these changes remains to be fully elucidated. We present the crystal structures of two truncated constructs of the LTTR DntR in their apo- forms and in complex with its natural inducer molecule, salicylate. These provide a fuller picture of the conformational changes that can occur in LTTR IBDs and offer insights that may be relevant when considering the mechanism of activation of LTTRs. Two of the crystal structures show that DntR IBDs can bind up to two inducer molecules. The full extent of conformational changes observed is achieved only when inducer molecules are bound in both binding sites identified. Point mutations disrupting the putative secondary binding site produce DntR variants with a reduced response to salicylate in a whole cell system, suggesting that this site is functionally relevant.

PubMed: 21692874
DOI: 10.1111/J.1365-2958.2011.07673.X

実験手法

X-RAY DIFFRACTION (1.95 Å)