2Y7C

Atomic model of the Ocr-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.

2Y7C の概要

• エントリーDOI: 10.2210/pdb2y7c/pdb
• 関連するPDBエントリー: 1S7Z 1YF2 2AR0 2Y7H
• EMDBエントリー: 1534
• 分子名称: TYPE-1 RESTRICTION ENZYME ECOKI SPECIFICITY PROTEIN, TYPE I RESTRICTION ENZYME ECOKI M PROTEIN, GENE 0.3 PROTEIN (3 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: ENTEROBACTERIA PHAGE T7; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 197604.64

構造登録者

Kennaway, C.K.,Obarska-Kosinska, A.,White, J.H.,Tuszynska, I.,Cooper, L.P.,Bujnicki, J.M.,Trinick, J.,Dryden, D.T.F. (登録日: 2011-01-31, 公開日: 2011-02-09, 最終更新日: 2024-05-08)

主引用文献

Kennaway, C.K.,Obarska-Kosinska, A.,White, J.H.,Tuszynska, I.,Cooper, L.P.,Bujnicki, J.M.,Trinick, J.,Dryden, D.T.F.
The Structure of M.Ecoki Type I DNA Methyltransferase with a DNA Mimic Antirestriction Protein.
Nucleic Acids Res., 37:762-, 2009

PubMed Abstract: Type-I DNA restriction-modification (R/M) systems are important agents in limiting the transmission of mobile genetic elements responsible for spreading bacterial resistance to antibiotics. EcoKI, a Type I R/M enzyme from Escherichia coli, acts by methylation- and sequence-specific recognition, leading to either methylation of DNA or translocation and cutting at a random site, often hundreds of base pairs away. Consisting of one specificity subunit, two modification subunits, and two DNA translocase/endonuclease subunits, EcoKI is inhibited by the T7 phage antirestriction protein ocr, a DNA mimic. We present a 3D density map generated by negative-stain electron microscopy and single particle analysis of the central core of the restriction complex, the M.EcoKI M(2)S(1) methyltransferase, bound to ocr. We also present complete atomic models of M.EcoKI in complex with ocr and its cognate DNA giving a clear picture of the overall clamp-like operation of the enzyme. The model is consistent with a large body of experimental data on EcoKI published over 40 years.

PubMed: 19074193
DOI: 10.1093/NAR/GKN988

実験手法

ELECTRON MICROSCOPY (18 Å)