2Y75

The Structure of CymR (YrzC) the Global Cysteine Regulator of B. subtilis

2Y75 の概要

• エントリーDOI: 10.2210/pdb2y75/pdb
• 分子名称: HTH-TYPE TRANSCRIPTIONAL REGULATOR CYMR, SULFATE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: transcription, dna binding protein
• 由来する生物種: BACILLUS SUBTILIS
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 86561.99

構造登録者

Shepard, W.,Soutourina, O.,Courtois, E.,England, P.,Haouz, A.,Martin-Verstraete, I. (登録日: 2011-01-28, 公開日: 2011-08-03, 最終更新日: 2024-05-08)

主引用文献

Shepard, W.,Soutourina, O.,Courtois, E.,England, P.,Haouz, A.,Martin-Verstraete, I.
Insights Into the Rrf2 Repressor Family - the Structure of Cymr, the Global Cysteine Regulator of Bacillus Subtilis.
FEBS J., 278:2689-, 2011

PubMed Abstract: The global regulator CymR represses the transcription of a large set of genes involved in cystine uptake and cysteine biosynthesis in Bacillus subtilis and Staphylococcus aureus. This repressor belongs to the widespread and poorly characterized Rrf2 family of regulators. The crystal structure of CymR from B. subtilis reveals a biologically active dimer, where each monomer folds into two tightly packed domains: a DNA-binding domain, which houses a winged helix-turn-helix (wHTH) motif; and a long dimerization domain, which places the wHTH motifs at the extremes. This architecture explains how these small regulators can span 23-27-bp DNA targets. The wHTH motif of CymR resembles those of the GntR superfamily of regulators, such as FadR and HutC. Superimposing the FadR wHTH motifs bound to their DNA fragments onto the wHTH motifs of the CymR dimer structure suggests that the DNA target and/or the protein must undergo some conformational changes upon binding. The CymR structure also hints at a possible location of the Fe-S centre associated with several Rrf2-type regulators.

PubMed: 21624051
DOI: 10.1111/J.1742-4658.2011.08195.X

実験手法

X-RAY DIFFRACTION (2 Å)