2Y5M

STRUCTURE OF LINEAR GRAMICIDIN D OBTAINED USING TYPE I CRYSTALS GROWN IN A 7.7 MONOACYLGLYCEROL LIPID CUBIC PHASE.

2Y5M の概要

• エントリーDOI: 10.2210/pdb2y5m/pdb
• 関連するPDBエントリー: 1AL4 1ALX 1ALZ 1AV2 1BDW 1C4D 1GMK 1GRM 1JNO 1JO3 1JO4 1KQE 1MAG 1MIC 1NG8 1NRM 1NRU 1NT5 1NT6 1TKQ 1W5U 2IZQ 2XDC 2Y6N
• 関連するBIRD辞書のPRD_ID: PRD_000150
• 分子名称: VAL-GRAMICIDIN A, POLYETHYLENE GLYCOL (N=34) (3 entities in total)
• 機能のキーワード: antibiotic, ion channel, mesophase, sponge phase
• 由来する生物種: BREVIBACILLUS BREVIS
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 15883.25

構造登録者

Hoefer, N.,Aragao, D.,Caffrey, M. (登録日: 2011-01-14, 公開日: 2011-05-11, 最終更新日: 2024-10-16)

主引用文献

Hoefer, N.,Aragao, D.,Lyons, J.,Caffrey, M.
Membrane Protein Crystallization in Lipidic Mesophases. Hosting Lipid Effects on the Crystallization and Structure of a Transmembrane Peptide
Cryst.Growth Des., 11:1182-, 2011

PubMed Abstract: Gramicidin is an apolar pentadecapeptide antibiotic consisting of alternating D-and L-amino acids. It functions, in part, by creating pores in membranes of susceptible cells rendering them leaky to monovalent cations. The peptide should be able to traverse the host membrane either as a double stranded, intertwined double helix (DSDH) or as a head-to-head single stranded helix (HHSH). Current structure models are based on macromolecular X-ray crystallography (MX) and nuclear magnetic resonance (NMR). However, the HHSH form has only been observed by NMR. The shape and size of the different gramicidin conformations differ. We speculated therefore that reconstituting it into a lipidic mesophase with bilayers of different microstructures would preferentially stabilize one form over the other. By using such mesophases for in meso crystallogenesis the expectation was that at least one would generate crystals of gramicidin in the HHSH form for structure determination by MX. This was tested using commercial and in-house synthesised lipids that support in meso crystallogenesis. Lipid acyl chain lengths were varied from 14 to 18 carbons to provide mesophases with a range of bilayer thicknesses. Unexpectedly, all lipids produced high quality, structure-grade crystals with gramicidin only in the DSDH conformation.

PubMed: 22933857
DOI: 10.1021/CG101384P

実験手法

X-RAY DIFFRACTION (1.08 Å)