2Y3M

Structure of the extra-membranous domain of the secretin HofQ from Actinobacillus actinomycetemcomitans

2Y3M の概要

• エントリーDOI: 10.2210/pdb2y3m/pdb
• 分子名称: PROTEIN TRANSPORT PROTEIN HOFQ, GLYCEROL, FORMIC ACID, ... (4 entities in total)
• 機能のキーワード: transport protein, secretin, dna uptake, competence
• 由来する生物種: AGGREGATIBACTER ACTINOMYCETEMCOMITANS
• 細胞内の位置: Cell outer membrane (By similarity): C9R226
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39621.32

構造登録者

Tarry, M.,Jaaskelainen, M.,Tuominen, H.,Ihalin, R.,Hogbom, M. (登録日: 2010-12-21, 公開日: 2011-04-27, 最終更新日: 2024-05-08)

主引用文献

Tarry, M.,Jaaskelainen, M.,Paino, A.,Tuominen, H.,Ihalin, R.,Hogbom, M.
The Extra-Membranous Domains of the Competence Protein Hofq Show DNA Binding, Flexibility and a Shared Fold with Type I Kh Domains.
J.Mol.Biol., 409:642-, 2011

PubMed Abstract: Secretins form large oligomeric assemblies in the membrane that control both macromolecular secretion and uptake. Several Pasteurellaceae are naturally competent for transformation, but the mechanism for DNA assimilation is largely unknown. In Haemophilus influenzae, the secretin ComE has been demonstrated to be essential for DNA uptake. In closely related Aggregatibacter actinomycetemcomitans, an opportunistic pathogen in periodontitis, the ComE homolog HofQ is believed to be the outer membrane DNA translocase. Here, we report the structure of the extra-membranous domains of HofQ at 2.3 Å resolution by X-ray crystallography. We also show that the extra-membranous domains of HofQ are capable of DNA binding. The structure reveals two secretin-like folds, the first of which is formed by means of a domain swap. The second domain displays extensive structural similarity to K homology (KH) domains, including the presence of a GxxG motif, which is essential for the nucleotide-binding function of KH domains, suggesting a possible mechanism for DNA binding by HofQ. The data indicate a direct involvement in DNA acquisition and provide insight into the molecular basis for natural competence.

PubMed: 21530539
DOI: 10.1016/J.JMB.2011.04.034

実験手法

X-RAY DIFFRACTION (2.3 Å)