2Y3H

E63Q mutant of Cupriavidus metallidurans CH34 CnrXs

Summary for 2Y3H

• Entry DOI: 10.2210/pdb2y3h/pdb
• Related: 2Y39 2Y3B 2Y3D 2Y3G
• Descriptor: NICKEL AND COBALT RESISTANCE PROTEIN CNRR, GLYCEROL (3 entities in total)
• Functional Keywords: metal binding protein
• Biological source: CUPRIAVIDUS METALLIDURANS
• Cellular location: Periplasm: P37975
• Total number of polymer chains: 4
• Total formula weight: 53611.93

Authors

Trepreau, J.,Girard, E.,Maillard, A.P.,de Rosny, E.,Petit-Haertlein, I.,Kahn, R.,Coves, J. (deposition date: 2010-12-20, release date: 2011-03-30, Last modification date: 2023-12-20)

Primary citation

Trepreau, J.,Girard, E.,Maillard, A.P.,De Rosny, E.,Petit-Haertlein, I.,Kahn, R.,Coves, J.
Structural Basis for Metal Sensing by Cnrx.
J.Mol.Biol., 408:766-, 2011

PubMed Abstract: CnrX is the metal sensor and signal modulator of the three-protein transmembrane signal transduction complex CnrYXH of Cupriavidus metallidurans CH34 that is involved in the setup of cobalt and nickel resistance. We have determined the atomic structure of the soluble domain of CnrX in its Ni-bound, Co-bound, or Zn-bound form. Ni and Co ions elicit a biological response, while the Zn-bound form is inactive. The structures presented here reveal the topology of intraprotomer and interprotomer interactions and the ability of metal-binding sites to fine-tune the packing of CnrX dimer as a function of the bound metal. These data suggest an allosteric mechanism to explain how the complex is switched on and how the signal is modulated by Ni or Co binding. These results provide clues to propose a model for signal propagation through the membrane in the complex.

PubMed: 21414325
DOI: 10.1016/J.JMB.2011.03.014

Experimental method

X-RAY DIFFRACTION (1.892 Å)