2Y1J

CRYSTAL STRUCTURE OF A R-DIASTEREOMER ANALOGUE OF THE SPORE PHOTOPRODUCT IN COMPLEX WITH FRAGMENT DNA POLYMERASE I FROM BACILLUS STEAROTHERMOPHILUS

Summary for 2Y1J

• Entry DOI: 10.2210/pdb2y1j/pdb
• Related: 2Y1I
• Descriptor: DNA POLYMERASE I, 5'-D(*GP*AP*CP*CP*AP*AP*CP*CP*CP*TP)-3', 5'-D(*AP*GP*GP*GP*QBTP*THM*GP*GP*TP*CP)-3', ... (5 entities in total)
• Functional Keywords: transferase-dna complex, dna repair, spore photoproduct, photolesion, uv lesion, 5-thyminyl-5, 6-dihydrothymine, transferase/dna
• Biological source: GEOBACILLUS STEAROTHERMOPHILUS; More
• Total number of polymer chains: 3
• Total formula weight: 72406.16

Authors

Heil, K.,Schneider, S.,Mueller, M.,Kneuttinger, A.C.,Carell, T. (deposition date: 2010-12-08, release date: 2011-07-27, Last modification date: 2023-12-20)

Primary citation

Heil, K.,Kneuttinger, A.C.,Schneider, S.,Lischke, U.,Carell, T.
Crystal Structures and Repair Studies Reveal the Identity and the Base-Pairing Properties of the Uv-Induced Spore Photoproduct DNA Lesion.
Chemistry, 17:9651-, 2011

PubMed Abstract: UV light is one of the major causes of DNA damage. In spore DNA, due to an unusual packing of the genetic material, a special spore photoproduct lesion (SP lesion) is formed, which is repaired by the enzyme spore photoproduct lyase (Spl), a radical S-adenosylmethionine (SAM) enzyme. We report here the synthesis and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester backbone. The oligonucleotides were used for repair studies and they were cocrystallized with a polymerase enzyme as a template to clarify the configuration of the SP lesion and to provide information about the base-pairing properties of the lesion. The structural analysis together with repair studies allowed us to clarify the identity of the preferentially repaired lesion diastereoisomer.

PubMed: 21780197
DOI: 10.1002/CHEM.201100177

Experimental method

X-RAY DIFFRACTION (2.15 Å)