2Y0S

Crystal structure of Sulfolobus shibatae RNA polymerase in P21 space group

2Y0S の概要

• エントリーDOI: 10.2210/pdb2y0s/pdb
• 分子名称: DNA-DIRECTED RNA POLYMERASE, RNA POLYMERASE SUBUNIT 13, DNA-DIRECTED RNA POLYMERASE SUBUNIT L, ... (16 entities in total)
• 機能のキーワード: transferase, nucleotidyltransferase, transcription, metal-binding
• 由来する生物種: SULFOLOBUS SHIBATAE; 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 812824.97

構造登録者

Wojtas, M.,Peralta, B.,Ondiviela, M.,Mogni, M.,Bell, S.D.,Abrescia, N.G.A. (登録日: 2010-12-07, 公開日: 2011-02-16, 最終更新日: 2024-11-06)

主引用文献

Wojtas, M.,Peralta, B.,Ondiviela, M.,Mogni, M.,Bell, S.D.,Abrescia, N.G.
Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit.
Biochem. Soc. Trans., 39:25-30, 2011

PubMed Abstract: We review recent results on the complete structure of the archaeal RNAP (RNA polymerase) enzyme of Sulfolobus shibatae. We compare the three crystal forms in which this RNAP packs (space groups P2₁2₁2₁, P2₁2₁2 and P2₁) and provide a preliminary biophysical characterization of the newly identified 13-subunit Rpo13. The availability of different crystal forms for this RNAP allows the analysis of the packing degeneracy and the intermolecular interactions that determine this degeneracy. We observe the pivotal role played by the protruding stalk composed of subunits Rpo4 and Rpo7 in the lattice contacts. Aided by MALLS (multi-angle laser light scattering), we have initiated the biophysical characterization of the recombinantly expressed and purified subunit Rpo13, a necessary step towards the understanding of Rpo13's role in archaeal transcription.

PubMed: 21265742
DOI: 10.1042/BST0390025

実験手法

X-RAY DIFFRACTION (3.8 Å)