2XZ2
Crystal structure of CstF-50 homodimerization domain
Summary for 2XZ2
| Entry DOI | 10.2210/pdb2xz2/pdb |
| Descriptor | CSTF-50, ISOFORM B, SODIUM ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total) |
| Functional Keywords | rna-binding protein, 3' end mrna maturation, transcription, rna binding protein |
| Biological source | DROSOPHILA MELANOGASTER (FRUIT FLY) |
| Total number of polymer chains | 1 |
| Total formula weight | 7846.14 |
| Authors | Moreno-Morcillo, M.,Fribourg, S. (deposition date: 2010-11-22, release date: 2011-01-26, Last modification date: 2024-05-08) |
| Primary citation | Moreno-Morcillo, M.,Minvielle-Sebastia, L.,Mackereth, C.,Fribourg, S. Hexameric Architecture of Cstf Supported by Cstf- 50 Homodimerization Domain Structure. RNA, 17:412-, 2011 Cited by PubMed Abstract: The Cleavage stimulation Factor (CstF) complex is composed of three subunits and is essential for pre-mRNA 3'-end processing. CstF recognizes U and G/U-rich cis-acting RNA sequence elements and helps stabilize the Cleavage and Polyadenylation Specificity Factor (CPSF) at the polyadenylation site as required for productive RNA cleavage. Here, we describe the crystal structure of the N-terminal domain of Drosophila CstF-50 subunit. It forms a compact homodimer that exposes two geometrically opposite, identical, and conserved surfaces that may serve as binding platform. Together with previous data on the structure of CstF-77, homodimerization of CstF-50 N-terminal domain supports the model in which the functional state of CstF is a heterohexamer. PubMed: 21233223DOI: 10.1261/RNA.2481011 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
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