2XZ2

Crystal structure of CstF-50 homodimerization domain

2XZ2 の概要

• エントリーDOI: 10.2210/pdb2xz2/pdb
• 分子名称: CSTF-50, ISOFORM B, SODIUM ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: rna-binding protein, 3' end mrna maturation, transcription, rna binding protein
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7846.14

構造登録者

Moreno-Morcillo, M.,Fribourg, S. (登録日: 2010-11-22, 公開日: 2011-01-26, 最終更新日: 2024-05-08)

主引用文献

Moreno-Morcillo, M.,Minvielle-Sebastia, L.,Mackereth, C.,Fribourg, S.
Hexameric Architecture of Cstf Supported by Cstf- 50 Homodimerization Domain Structure.
RNA, 17:412-, 2011

PubMed Abstract: The Cleavage stimulation Factor (CstF) complex is composed of three subunits and is essential for pre-mRNA 3'-end processing. CstF recognizes U and G/U-rich cis-acting RNA sequence elements and helps stabilize the Cleavage and Polyadenylation Specificity Factor (CPSF) at the polyadenylation site as required for productive RNA cleavage. Here, we describe the crystal structure of the N-terminal domain of Drosophila CstF-50 subunit. It forms a compact homodimer that exposes two geometrically opposite, identical, and conserved surfaces that may serve as binding platform. Together with previous data on the structure of CstF-77, homodimerization of CstF-50 N-terminal domain supports the model in which the functional state of CstF is a heterohexamer.

PubMed: 21233223
DOI: 10.1261/RNA.2481011

実験手法

X-RAY DIFFRACTION (1.4 Å)