2XWX

Vibrio cholerae colonization factor GbpA crystal structure

2XWX の概要

• エントリーDOI: 10.2210/pdb2xwx/pdb
• 分子名称: GLCNAC-BINDING PROTEIN A (2 entities in total)
• 機能のキーワード: chitin-binding protein
• 由来する生物種: VIBRIO CHOLERAE
• 細胞内の位置: Secreted : Q9KLD5
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86585.97

構造登録者

Wong, E.,Vaaje-Kolstad, G.,Ghosh, A.,Guerrero, R.H.,Konarev, P.V.,Ibrahim, A.F.M.,Svergun, D.I.,Eijsink, V.G.H.,Chatterjee, N.S.,van Aalten, D.M.F. (登録日: 2010-11-06, 公開日: 2011-11-16, 最終更新日: 2024-11-13)

主引用文献

Wong, E.,Vaaje-Kolstad, G.,Ghosh, A.,Hurtado-Guerrero, R.,Konarev, P.V.,Ibrahim, A.F.M.,Svergun, D.I.,Eijsink, V.G.H.,Chatterjee, N.S.,Van Aalten, D.M.F.
The Vibrio Cholerae Colonization Factor Gbpa Possesses a Modular Structure that Governs Binding to Different Host Surfaces.
Plos Pathog., 8:2373-, 2012

PubMed Abstract: Vibrio cholerae is a bacterial pathogen that colonizes the chitinous exoskeleton of zooplankton as well as the human gastrointestinal tract. Colonization of these different niches involves an N-acetylglucosamine binding protein (GbpA) that has been reported to mediate bacterial attachment to both marine chitin and mammalian intestinal mucin through an unknown molecular mechanism. We report structural studies that reveal that GbpA possesses an unusual, elongated, four-domain structure, with domains 1 and 4 showing structural homology to chitin binding domains. A glycan screen revealed that GbpA binds to GlcNAc oligosaccharides. Structure-guided GbpA truncation mutants show that domains 1 and 4 of GbpA interact with chitin in vitro, whereas in vivo complementation studies reveal that domain 1 is also crucial for mucin binding and intestinal colonization. Bacterial binding studies show that domains 2 and 3 bind to the V. cholerae surface. Finally, mouse virulence assays show that only the first three domains of GbpA are required for colonization. These results explain how GbpA provides structural/functional modular interactions between V. cholerae, intestinal epithelium and chitinous exoskeletons.

PubMed: 22253590
DOI: 10.1371/JOURNAL.PPAT.1002373

実験手法

X-RAY DIFFRACTION (1.8 Å)