2XVV

Human serum albumin complexed with dansyl-L-asparagine and myristic acid

Summary for 2XVV

• Entry DOI: 10.2210/pdb2xvv/pdb
• Related: 1AO6 1BJ5 1BKE 1BM0 1E78 1E7A 1E7B 1E7C 1E7E 1E7F 1E7G 1E7H 1E7I 1GNI 1GNJ 1H9Z 1HA2 1HK1 1HK2 1HK3 1HK4 1HK5 1N5U 1O9X 1TF0 1UOR 1YSX 2BX8 2BXA 2BXB 2BXC 2BXD 2BXE 2BXF 2BXG 2BXH 2BXI 2BXK 2BXL 2BXM 2BXN 2BXO 2BXP 2BXQ 2ESG 2VDB 2VUE 2VUF 2XSI 2XVQ 2XVU 2XVW 2XW0 2XW1
• Descriptor: SERUM ALBUMIN, MYRISTIC ACID, DANSYL-L-ASPARAGINE, ... (4 entities in total)
• Functional Keywords: transport protein
• Biological source: HOMO SAPIENS (HUMAN)
• Cellular location: Secreted: P02768
• Total number of polymer chains: 1
• Total formula weight: 68644.18

Authors

Ghuman, J.,Curry, S. (deposition date: 2010-10-28, release date: 2010-11-10, Last modification date: 2023-12-20)

Primary citation

Ryan, A.J.,Ghuman, J.,Zunszain, P.A.,Chung, C.W.,Curry, S.
Structural Basis of Binding of Fluorescent, Site-Specific Dansylated Amino Acids to Human Serum Albumin.
J.Struct.Biol., 174:84-, 2011

PubMed Abstract: Human serum albumin (HSA) has two primary binding sites for drug molecules. These sites selectively bind different dansylated amino acid compounds, which-due to their intrinsic fluorescence-have long been used as specific markers for the drug pockets on HSA. We present here the co-crystal structures of HSA in complex with six dansylated amino acids that are specific for either drug site 1 (dansyl-l-asparagine, dansyl-l-arginine, dansyl-l-glutamate) or drug site 2 (dansyl-l-norvaline, dansyl-l-phenylalanine, dansyl-l-sarcosine). Our results explain the structural basis of the site-specificity of different dansylated amino acids. They also show that fatty acid binding has only a modest effect on binding of dansylated amino acids to drug site 1 and identify the location of secondary binding sites.

PubMed: 20940056
DOI: 10.1016/J.JSB.2010.10.004

Experimental method

X-RAY DIFFRACTION (2.4 Å)