2XTL

Structure of the major pilus backbone protein from Streptococcus Agalactiae

2XTL の概要

• エントリーDOI: 10.2210/pdb2xtl/pdb
• 分子名称: CELL WALL SURFACE ANCHOR FAMILY PROTEIN, POTASSIUM ION (3 entities in total)
• 機能のキーワード: gram-positive pili protein, bp-2a 515 allele, immunoglobulin-like domains, structural protein
• 由来する生物種: STREPTOCOCCUS AGALACTIAE
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 98943.15

構造登録者

Rinauda, D.,Gourlay, L.J.,Soriano, M.,Grandi, G.,Bolognesi, M. (登録日: 2010-10-11, 公開日: 2011-07-06, 最終更新日: 2024-10-23)

主引用文献

Nuccitelli, A.,Cozzi, R.,Gourlay, L.J.,Donnarumma, D.,Necchi, F.,Norais, N.,Telford, J.L.,Rappuoli, R.,Bolognesi, M.,Maione, D.,Grandi, G.,Rinaudo, C.D.
Structure-Based Approach to Rationally Design a Chimeric Protein for an Effective Vaccine Against Group B Streptococcus Infections.
Proc.Natl.Acad.Sci.USA, 108:10278-, 2011

PubMed Abstract: Structural vaccinology is an emerging strategy for the rational design of vaccine candidates. We successfully applied structural vaccinology to design a fully synthetic protein with multivalent protection activity. In Group B Streptococcus, cell-surface pili have aroused great interest because of their direct roles in virulence and importance as protective antigens. The backbone subunit of type 2a pilus (BP-2a) is present in six immunogenically different but structurally similar variants. We determined the 3D structure of one of the variants, and experimentally demonstrated that protective antibodies specifically recognize one of the four domains that comprise the protein. We therefore constructed a synthetic protein constituted by the protective domain of each one of the six variants and showed that the chimeric protein protects mice against the challenge with all of the type 2a pilus-carrying strains. This work demonstrates the power of structural vaccinology and will facilitate the development of an optimized, broadly protective pilus-based vaccine against Group B Streptococcus by combining the uniquely generated chimeric protein with protective pilin subunits from two other previously identified pilus types. In addition, this work describes a template procedure that can be followed to develop vaccines against other bacterial pathogens.

PubMed: 21593422
DOI: 10.1073/PNAS.1106590108

実験手法

X-RAY DIFFRACTION (1.75 Å)