2XQ8

Pentameric ligand gated ion channel GLIC in complex with zinc ion (Zn2+)

2XQ8 の概要

• エントリーDOI: 10.2210/pdb2xq8/pdb
• 関連するPDBエントリー: 2XQ3 2XQ4 2XQ5 2XQ6 2XQ7 2XQ9 2XQA
• 分子名称: GLR4197 PROTEIN, ZINC ION (2 entities in total)
• 機能のキーワード: membrane protein, open channel block
• 由来する生物種: GLOEOBACTER VIOLACEUS PCC 7421
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 181584.84

構造登録者

Hilf, R.J.C.,Bertozzi, C.,Zimmermann, I.,Reiter, A.,Trauner, D.,Dutzler, R. (登録日: 2010-09-01, 公開日: 2010-11-10, 最終更新日: 2024-05-08)

主引用文献

Hilf, R.J.C.,Bertozzi, C.,Zimmermann, I.,Reiter, A.,Trauner, D.,Dutzler, R.
Structural Basis of Open Channel Block in a Prokaryotic Pentameric Ligand-Gated Ion Channel
Nat.Struct.Mol.Biol., 17:1330-, 2010

PubMed Abstract: The flow of ions through cation-selective members of the pentameric ligand-gated ion channel family is inhibited by a structurally diverse class of molecules that bind to the transmembrane pore in the open state of the protein. To obtain insight into the mechanism of channel block, we have investigated the binding of positively charged inhibitors to the open channel of the bacterial homolog GLIC by using X-ray crystallography and electrophysiology. Our studies reveal the location of two regions for interactions, with larger blockers binding in the center of the membrane and divalent transition metal ions binding to the narrow intracellular pore entry. The results provide a structural foundation for understanding the interactions of the channel with inhibitors that is relevant for the entire family.

PubMed: 21037567
DOI: 10.1038/NSMB.1933

実験手法

X-RAY DIFFRACTION (3.6 Å)