2XPN

Crystal structure of a Spt6-Iws1(Spn1) complex from Encephalitozoon cuniculi, Form I

2XPN の概要

• エントリーDOI: 10.2210/pdb2xpn/pdb
• 関連するPDBエントリー: 2XPL 2XPO 2XPP
• 分子名称: IWS1, CHROMATIN STRUCTURE MODULATOR, BROMIDE ION, ... (4 entities in total)
• 機能のキーワード: transcription, elongation, histone chaperone, rna polymerase ii, mrna export
• 由来する生物種: ENCEPHALITOZOON CUNICULI; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19216.95

構造登録者

Diebold, M.-L.,Koch, M.,Cura, V.,Cavarelli, J.,Romier, C. (登録日: 2010-08-27, 公開日: 2010-11-17, 最終更新日: 2023-12-20)

主引用文献

Diebold, M.-L.,Koch, M.,Loeliger, E.,Cura, V.,Winston, F.,Cavarelli, J.,Romier, C.
The Structure of an Iws1/Spt6 Complex Reveals an Interaction Domain Conserved in Tfiis, Elongin a and Med26
Embo J., 29:3979-, 2010

PubMed Abstract: Binding of elongation factor Spt6 to Iws1 provides an effective means for coupling eukaryotic mRNA synthesis, chromatin remodelling and mRNA export. We show that an N-terminal region of Spt6 (Spt6N) is responsible for interaction with Iws1. The crystallographic structures of Encephalitozoon cuniculi Iws1 and the Iws1/Spt6N complex reveal two conserved binding subdomains in Iws1. The first subdomain (one HEAT repeat; HEAT subdomain) is a putative phosphoprotein-binding site most likely involved in an Spt6-independent function of Iws1. The second subdomain (two ARM repeats; ARM subdomain) specifically recognizes a bipartite N-terminal region of Spt6. Mutations that alter this region of Spt6 cause severe phenotypes in vivo. Importantly, the ARM subdomain of Iws1 is conserved in several transcription factors, including TFIIS, Elongin A and Med26. We show that the homologous region in yeast TFIIS enables this factor to interact with SAGA and the Mediator subunits Spt8 and Med13, suggesting the molecular basis for TFIIS recruitment at promoters. Taken together, our results provide new structural information about the Iws1/Spt6 complex and reveal a novel interaction domain used for the formation of transcription networks.

PubMed: 21057455
DOI: 10.1038/EMBOJ.2010.272

実験手法

X-RAY DIFFRACTION (1.95 Å)