2XON

Structure of TmCBM61 in complex with beta-1,4-galactotriose at 1.4 A resolution

2XON の概要

• エントリーDOI: 10.2210/pdb2xon/pdb
• 関連するPDBエントリー: 2XOM
• 関連するBIRD辞書のPRD_ID: PRD_900114
• 分子名称: ARABINOGALACTAN ENDO-1,4-BETA-GALACTOSIDASE, beta-D-galactopyranose-(1-4)-beta-D-galactopyranose-(1-4)-beta-D-galactopyranose, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: hydrolase, galactan, carbohydrate-binding module, glycoside hydrolase, beta-sandwich.
• 由来する生物種: THERMOTOGA MARITIMA
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37269.05

構造登録者

Cid, M.,Lodberg-Pedersen, H.,Kaneko, S.,Coutinho, P.M.,Henrissat, B.,Willats, W.G.T.,Boraston, A.B. (登録日: 2010-08-20, 公開日: 2010-09-08, 最終更新日: 2023-12-20)

主引用文献

Cid, M.,Pedersen, H.L.,Kaneko, S.,Coutinho, P.M.,Henrissat, B.,Willats, W.G.T.,Boraston, A.B.
Recognition of the Helical Structure of Beta-1,4-Galactan by a New Family of Carbohydrate-Binding Modules.
J.Biol.Chem., 285:35999-, 2010

PubMed Abstract: The microbial enzymes that depolymerize plant cell wall polysaccharides, ultimately promoting energy liberation and carbon recycling, are typically complex in their modularity and often contain carbohydrate-binding modules (CBMs). Here, through analysis of an unknown module from a Thermotoga maritima endo-β-1,4-galactanase, we identify a new family of CBMs that are most frequently found appended to proteins with β-1,4-galactanase activity. Polysaccharide microarray screening, immunofluorescence microscopy, and biochemical analysis of the isolated module demonstrate the specificity of the module, here called TmCBM61, for β-1,4-linked galactose-containing ligands, making it the founding member of family CBM61. The ultra-high resolution X-ray crystal structures of TmCBM61 (0.95 and 1.4 Å resolution) in complex with β-1,4-galactotriose reveal the molecular basis of the specificity of the CBM for β-1,4-galactan. Analysis of these structures provides insight into the recognition of an unexpected helical galactan conformation through a mode of binding that resembles the recognition of starch.

PubMed: 20826814
DOI: 10.1074/JBC.M110.166330

実験手法

X-RAY DIFFRACTION (1.4 Å)