2XKK

CRYSTAL STRUCTURE OF MOXIFLOXACIN, DNA, and A. BAUMANNII TOPO IV (PARE-PARC FUSION TRUNCATE)

2XKK の概要

• エントリーDOI: 10.2210/pdb2xkk/pdb
• 関連するPDBエントリー: 2XKJ
• 分子名称: TOPOISOMERASE IV, DNA, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: isomerase-dna-antibiotic complex, type iia topoisomerase, quinolone, antibacterial agent, isomerase/dna/antibiotic
• 由来する生物種: ACINETOBACTER BAUMANNII; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 194147.10

構造登録者

Wohlkonig, A.,Chan, P.F.,Fosberry, A.P.,Homes, P.,Huang, J.,Kranz, M.,Leydon, V.R.,Miles, T.J.,Pearson, N.D.,Perera, R.L.,Shillings, A.J.,Gwynn, M.N.,Bax, B.D. (登録日: 2010-07-08, 公開日: 2010-09-01, 最終更新日: 2024-11-06)

主引用文献

Wohlkonig, A.,Chan, P.F.,Fosberry, A.P.,Homes, P.,Huang, J.,Kranz, M.,Leydon, V.R.,Miles, T.J.,Pearson, N.D.,Perera, R.L.,Shillings, A.J.,Gwynn, M.N.,Bax, B.D.
Structural Basis of Quinolone Inhibition of Type Iia Topoisomerases and Target-Mediated Resistance
Nat.Struct.Mol.Biol., 17:1152-, 2010

PubMed Abstract: Quinolone antibacterials have been used to treat bacterial infections for over 40 years. A crystal structure of moxifloxacin in complex with Acinetobacter baumannii topoisomerase IV now shows the wedge-shaped quinolone stacking between base pairs at the DNA cleavage site and binding conserved residues in the DNA cleavage domain through chelation of a noncatalytic magnesium ion. This provides a molecular basis for the quinolone inhibition mechanism, resistance mutations and invariant quinolone antibacterial structural features.

PubMed: 20802486
DOI: 10.1038/NSMB.1892

実験手法

X-RAY DIFFRACTION (3.25 Å)