2XKA

Crystal structure of a GTPyS-form protofilament of Bacillus thuringiensis serovar israelensis TubZ

2XKA の概要

• エントリーDOI: 10.2210/pdb2xka/pdb
• 関連するPDBエントリー: 2XKB
• 分子名称: FTSZ/TUBULIN-RELATED PROTEIN, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: structural protein, motor protein, cytoskeleton, cytomotive, dna segregation, microtubule, pbtoxis, pbt156, repx, tubr
• 由来する生物種: BACILLUS THURINGIENSIS
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 335255.21

構造登録者

Aylett, C.H.S.,Lowe, J. (登録日: 2010-07-07, 公開日: 2010-11-10, 最終更新日: 2024-05-08)

主引用文献

Aylett, C.H.,Wang, Q.,Michie, K.A.,Amos, L.A.,Lowe, J.
Filament Structure of Bacterial Tubulin Homologue Tubz.
Proc.Natl.Acad.Sci.USA, 107:19766-, 2010

PubMed Abstract: Low copy number plasmids often depend on accurate partitioning systems for their continued survival. Generally, such systems consist of a centromere-like region of DNA, a DNA-binding adaptor, and a polymerizing cytomotive filament. Together these components drive newly replicated plasmids to opposite ends of the dividing cell. The Bacillus thuringiensis plasmid pBToxis relies on a filament of the tubulin/FtsZ-like protein TubZ for its segregation. By combining crystallography and electron microscopy, we have determined the structure of this filament. We explain how GTP hydrolysis weakens the subunit-subunit contact and also shed light on the partitioning of the plasmid-adaptor complex. The double helical superstructure of TubZ filaments is unusual for tubulin-like proteins. Filaments of ParM, the actin-like partitioning protein, are also double helical. We suggest that convergent evolution shapes these different types of cytomotive filaments toward a general mechanism for plasmid separation.

PubMed: 20974911
DOI: 10.1073/PNAS.1010176107

実験手法

X-RAY DIFFRACTION (3 Å)