2XJT

X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and Man5(D1)

2XJT の概要

• エントリーDOI: 10.2210/pdb2xjt/pdb
• 関連するPDBエントリー: 2XJP 2XJQ 2XJR 2XJS 2XJU 2XJV
• 分子名称: FLOCCULATION PROTEIN FLO5, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose, CALCIUM ION, ... (7 entities in total)
• 機能のキーワード: cell adhesion, greenbeard, social interaction, pa14-domain, carbohydrate binding
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28582.73

構造登録者

Veelders, M.,Brueckner, S.,Ott, D.,Unverzagt, C.,Moesch, H.-U.,Essen, L.-O. (登録日: 2010-07-06, 公開日: 2010-12-15, 最終更新日: 2024-11-20)

主引用文献

Veelders, M.,Brueckner, S.,Ott, D.,Unverzagt, C.,Moesch, H.-U.,Essen, L.-O.
Structural Basis of Flocculin-Mediated Social Behavior in Yeast
Proc.Natl.Acad.Sci.USA, 107:22511-, 2010

PubMed Abstract: In the budding yeast Saccharomyces cerevisiae, self-recognition and the thereby promoted aggregation of thousands of cells into protective flocs is mediated by a family of cell-surface adhesins, the flocculins (Flo). Based on this social behavior FLO genes fulfill the definition of "greenbeard" genes, which direct cooperation toward other carriers of the same gene. The process of flocculation plays an eminent role in the food industry for the production of beer and wine. However, the precise mode of flocculin-mediated surface recognition and the exact structure of cognate ligands have remained elusive. Here, we present structures of the adhesion domain of a flocculin complexed to its cognate ligands derived from yeast high-mannose oligosaccharides at resolutions up to 0.95 Å. Besides a PA14-like architecture, the Flo5A domain reveals a previously undescribed lectin fold that utilizes a unique DcisD calcium-binding motif for carbohydrate binding and that is widely spread among pro- and eukaryotes. Given the high abundance of high-mannose oligosaccharides in yeast cell walls, the Flo5A structure suggests a model for recognition, where social non-self- instead of unsocial self-interactions are favored.

PubMed: 21149680
DOI: 10.1073/PNAS.1013210108

実験手法

X-RAY DIFFRACTION (1.2 Å)