2XEA

4.6 ANGSTROM CRYO-EM RECONSTRUCTION OF TOBACCO MOSAIC VIRUS FROM IMAGES RECORDED AT 300 KEV ON A 4KX4K CCD CAMERA

Summary for 2XEA

Entry DOI	10.2210/pdb2xea/pdb
Related	2OM3
EMDB information	1730
Descriptor	TOBACCO MOSAIC VIRUS, 5'-(GPAP*AP)-3' (2 entities in total)
Functional Keywords	virus, single particle processing
Biological source	TOBACCO MOSAIC VIRUS More
Total number of polymer chains	2
Total formula weight	18464.09
Authors	Clare, D.K.,Orlova, E.V. (deposition date: 2010-05-12, release date: 2010-06-30, Last modification date: 2024-05-08)
Primary citation	Clare, D.K.,Orlova, E.V. 4.6 A Cryo-Em Reconstruction of Tobacco Mosaic Virus from Images Recorded at 300 Kev on a 4Kx4K Ccd Camera. J.Struct.Biol., 171:303-, 2010 Cited by PubMed Abstract: Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV. PubMed: 20558300 DOI: 10.1016/J.JSB.2010.06.011 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (4.6 Å)

Structure validation