2XEA

4.6 ANGSTROM CRYO-EM RECONSTRUCTION OF TOBACCO MOSAIC VIRUS FROM IMAGES RECORDED AT 300 KEV ON A 4KX4K CCD CAMERA

2XEA の概要

• エントリーDOI: 10.2210/pdb2xea/pdb
• 関連するPDBエントリー: 2OM3
• EMDBエントリー: 1730
• 分子名称: TOBACCO MOSAIC VIRUS, 5'-(*GP*AP*AP)-3' (2 entities in total)
• 機能のキーワード: virus, single particle processing
• 由来する生物種: TOBACCO MOSAIC VIRUS; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 18464.09

構造登録者

Clare, D.K.,Orlova, E.V. (登録日: 2010-05-12, 公開日: 2010-06-30, 最終更新日: 2024-05-08)

主引用文献

Clare, D.K.,Orlova, E.V.
4.6 A Cryo-Em Reconstruction of Tobacco Mosaic Virus from Images Recorded at 300 Kev on a 4Kx4K Ccd Camera.
J.Struct.Biol., 171:303-, 2010

PubMed Abstract: Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV.

PubMed: 20558300
DOI: 10.1016/J.JSB.2010.06.011

実験手法

ELECTRON MICROSCOPY (4.6 Å)