2XDY

Crystal structure of the N. crassa QDE-2 AGO MID domain

2XDY の概要

• エントリーDOI: 10.2210/pdb2xdy/pdb
• 分子名称: POST-TRANSCRIPTIONAL GENE SILENCING PROTEIN QDE-2, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: argonaute, gene silencing, rna-induced silencing complex, 5' end binding, rossmann-like fold, rna binding protein
• 由来する生物種: NEUROSPORA CRASSA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16263.00

構造登録者

Boland, A.,Tritschler, F.,Weichenrieder, O. (登録日: 2010-05-10, 公開日: 2010-06-16, 最終更新日: 2024-05-08)

主引用文献

Boland, A.,Tritschler, F.,Heimstaedt, S.,Izaurralde, E.,Weichenrieder, O.
Crystal Structure and Ligand Binding of the Mid Domain of a Eukaryotic Argonaute Protein.
Embo Rep., 11:522-, 2010

PubMed Abstract: Argonaute (AGO) proteins are core components of RNA-induced silencing complexes and have essential roles in RNA-mediated gene silencing. They are characterized by a bilobal architecture, consisting of one lobe containing the amino-terminal and PAZ domains and another containing the MID and PIWI domains. Except for the PAZ domain, structural information on eukaryotic AGO domains is not yet available. In this study, we report the crystal structure of the MID domain of the eukaryotic AGO protein QDE-2 from Neurospora crassa. This domain adopts a Rossmann-like fold and recognizes the 5'-terminal nucleotide of a guide RNA in a manner similar to its prokaryotic counterparts. The 5'-nucleotide-binding site shares common residues with a second, adjacent ligand-binding site, suggesting a mechanism for the cooperative binding of ligands to the MID domain of eukaryotic AGOs.

PubMed: 20539312
DOI: 10.1038/EMBOR.2010.81

実験手法

X-RAY DIFFRACTION (2.2 Å)