2XDD

A processed non-coding RNA regulates a bacterial antiviral system

2XDD の概要

• エントリーDOI: 10.2210/pdb2xdd/pdb
• 関連するPDBエントリー: 2XD0 2XDB
• 分子名称: TOXN, TOXI, ZINC ION, ... (4 entities in total)
• 機能のキーワード: toxin-rna complex, abortive infection, phage, toxin, toxin/rna
• 由来する生物種: PECTOBACTERIUM ATROSEPTICUM; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 95395.32

構造登録者

Blower, T.R.,Pei, X.Y.,Short, F.L.,Fineran, P.C.,Humphreys, D.P.,Luisi, B.F.,Salmond, G.P.C. (登録日: 2010-04-30, 公開日: 2011-01-12, 最終更新日: 2024-11-06)

主引用文献

Fineran, P.C.,Blower, T.R.,Foulds, I.J.,Humphreys, D.P.,Lilley, K.S.,Salmond, G.P.C.
The Phage Abortive Infection System, Toxin, Functions as a Protein-RNA Toxin-Antitoxin Pair.
Proc.Natl.Acad.Sci.USA, 106:894-, 2009

PubMed Abstract: Various mechanisms exist that enable bacteria to resist bacteriophage infection. Resistance strategies include the abortive infection (Abi) systems, which promote cell death and limit phage replication within a bacterial population. A highly effective 2-gene Abi system from the phytopathogen Erwinia carotovora subspecies atroseptica, designated ToxIN, is described. The ToxIN Abi system also functions as a toxin-antitoxin (TA) pair, with ToxN inhibiting bacterial growth and the tandemly repeated ToxI RNA antitoxin counteracting the toxicity. TA modules are currently divided into 2 classes, protein and RNA antisense. We provide evidence that ToxIN defines an entirely new TA class that functions via a novel protein-RNA mechanism, with analogous systems present in diverse bacteria. Despite the debated role of TA systems, we demonstrate that ToxIN provides viral resistance in a range of bacterial genera against multiple phages. This is the first demonstration of a novel mechanistic class of TA systems and of an Abi system functioning in different bacterial genera, both with implications for the dynamics of phage-bacterial interactions.

PubMed: 19124776
DOI: 10.1073/PNAS.0808832106

実験手法

X-RAY DIFFRACTION (3.2 Å)