2XD0
A processed non-coding RNA regulates a bacterial antiviral system
Summary for 2XD0
| Entry DOI | 10.2210/pdb2xd0/pdb |
| Related | 2XDB 2XDD |
| Descriptor | TOXN, TOXI (3 entities in total) |
| Functional Keywords | toxin-rna complex, abortive infection, phage, toxin/rna |
| Biological source | PECTOBACTERIUM ATROSEPTICUM More |
| Total number of polymer chains | 12 |
| Total formula weight | 187813.96 |
| Authors | Blower, T.R.,Pei, X.Y.,Short, F.L.,Fineran, P.C.,Humphreys, D.P.,Luisi, B.F.,Salmond, G.P.C. (deposition date: 2010-04-28, release date: 2011-01-12, Last modification date: 2024-05-01) |
| Primary citation | Blower, T.R.,Pei, X.Y.,Short, F.L.,Fineran, P.C.,Humphreys, D.P.,Luisi, B.F.,Salmond, G.P. A processed noncoding RNA regulates an altruistic bacterial antiviral system. Nat. Struct. Mol. Biol., 18:185-190, 2011 Cited by PubMed Abstract: The ≥ 10³⁰ bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanisms for this toxicity and inhibition have not been defined. Here we present the crystal structure of the ToxN-ToxI complex from Pectobacterium atrosepticum, determined to 2.75-Å resolution. ToxI is a 36-nucleotide noncoding RNA pseudoknot, and three ToxI monomers bind to three ToxN monomers to generate a trimeric ToxN-ToxI complex. Assembly of this complex is mediated entirely through extensive RNA-protein interactions. Furthermore, a 2'-3' cyclic phosphate at the 3' end of ToxI, and catalytic residues, identify ToxN as an endoRNase that processes ToxI from a repetitive precursor but is regulated by its own catalytic product. PubMed: 21240270DOI: 10.1038/nsmb.1981 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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