2XD0

A processed non-coding RNA regulates a bacterial antiviral system

2XD0 の概要

• エントリーDOI: 10.2210/pdb2xd0/pdb
• 関連するPDBエントリー: 2XDB 2XDD
• 分子名称: TOXN, TOXI (3 entities in total)
• 機能のキーワード: toxin-rna complex, abortive infection, phage, toxin/rna
• 由来する生物種: PECTOBACTERIUM ATROSEPTICUM; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 187813.96

構造登録者

Blower, T.R.,Pei, X.Y.,Short, F.L.,Fineran, P.C.,Humphreys, D.P.,Luisi, B.F.,Salmond, G.P.C. (登録日: 2010-04-28, 公開日: 2011-01-12, 最終更新日: 2024-05-01)

主引用文献

Blower, T.R.,Pei, X.Y.,Short, F.L.,Fineran, P.C.,Humphreys, D.P.,Luisi, B.F.,Salmond, G.P.
A processed noncoding RNA regulates an altruistic bacterial antiviral system.
Nat. Struct. Mol. Biol., 18:185-190, 2011

PubMed Abstract: The ≥ 10³⁰ bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanisms for this toxicity and inhibition have not been defined. Here we present the crystal structure of the ToxN-ToxI complex from Pectobacterium atrosepticum, determined to 2.75-Å resolution. ToxI is a 36-nucleotide noncoding RNA pseudoknot, and three ToxI monomers bind to three ToxN monomers to generate a trimeric ToxN-ToxI complex. Assembly of this complex is mediated entirely through extensive RNA-protein interactions. Furthermore, a 2'-3' cyclic phosphate at the 3' end of ToxI, and catalytic residues, identify ToxN as an endoRNase that processes ToxI from a repetitive precursor but is regulated by its own catalytic product.

PubMed: 21240270
DOI: 10.1038/nsmb.1981

実験手法

X-RAY DIFFRACTION (3 Å)