2XCY

Crystal structure of Aspergillus fumigatus sialidase

2XCY の概要

• エントリーDOI: 10.2210/pdb2xcy/pdb
• 分子名称: EXTRACELLULAR SIALIDASE/NEURAMINIDASE, PUTATIVE, CHLORIDE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: hydrolase, glycosidase
• 由来する生物種: ASPERGILLUS FUMIGATUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84867.35

構造登録者

Telford, J.C.,Yeung, J.,Xu, G.,Bennet, A.,Moore, M.M.,Taylor, G.L. (登録日: 2010-04-27, 公開日: 2010-05-12, 最終更新日: 2024-11-13)

主引用文献

Telford, J.C.,Yeung, J.H.,Xu, G.,Kiefel, M.J.,Watts, A.G.,Hader, S.,Chan, J.,Bennet, A.J.,Moore, M.M.,Taylor, G.L.
The Aspergillus Fumigatus Sialidase is a Kdnase: Structural and Mechanistic Insights.
J.Biol.Chem., 286:10783-, 2011

PubMed Abstract: Aspergillus fumigatus is a filamentous fungus that can cause severe respiratory disease in immunocompromised individuals. A putative sialidase from A. fumigatus was recently cloned and shown to be relatively poor in cleaving N-acetylneuraminic acid (Neu5Ac) in comparison with bacterial sialidases. Here we present the first crystal structure of a fungal sialidase. When the apo structure was compared with bacterial sialidase structures, the active site of the Aspergillus enzyme suggested that Neu5Ac would be a poor substrate because of a smaller pocket that normally accommodates the acetamido group of Neu5Ac in sialidases. A sialic acid with a hydroxyl in place of an acetamido group is 2-keto-3-deoxynononic acid (KDN). We show that KDN is the preferred substrate for the A. fumigatus sialidase and that A. fumigatus can utilize KDN as a sole carbon source. A 1.45-Å resolution crystal structure of the enzyme in complex with KDN reveals KDN in the active site in a boat conformation and nearby a second binding site occupied by KDN in a chair conformation, suggesting that polyKDN may be a natural substrate. The enzyme is not inhibited by the sialidase transition state analog 2-deoxy-2,3-dehydro-N-acetylneuraminic acid (Neu5Ac2en) but is inhibited by the related 2,3-didehydro-2,3-dideoxy-D-glycero-D-galacto-nonulosonic acid that we show bound to the enzyme in a 1.84-Å resolution crystal structure. Using a fluorinated KDN substrate, we present a 1.5-Å resolution structure of a covalently bound catalytic intermediate. The A. fumigatus sialidase is therefore a KDNase with a similar catalytic mechanism to Neu5Ac exosialidases, and this study represents the first structure of a KDNase.

PubMed: 21247893
DOI: 10.1074/JBC.M110.207043

実験手法

X-RAY DIFFRACTION (1.84 Å)