2XB2

Crystal structure of the core Mago-Y14-eIF4AIII-Barentsz-UPF3b assembly shows how the EJC is bridged to the NMD machinery

2XB2 の概要

• エントリーDOI: 10.2210/pdb2xb2/pdb
• 関連するPDBエントリー: 1P27 1UW4 2J0Q 2J0S 2J0U
• 分子名称: EUKARYOTIC INITIATION FACTOR 4A-III, PROTEIN MAGO NASHI HOMOLOG, RNA-BINDING PROTEIN 8A, ... (9 entities in total)
• 機能のキーワード: exon junction complex, nonsense-mediated mrna decay, translation, upf3b, hydrolase
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: P38919 P61326 Q9Y5S9 Q9BZI7; Cytoplasm, perinuclear region: O15234
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 208445.59

構造登録者

Buchwald, G.,Ebert, J.,Basquin, C.,Sauliere, J.,Jayachandran, U.,Bono, F.,Le Hir, H.,Conti, E. (登録日: 2010-04-03, 公開日: 2010-05-12, 最終更新日: 2023-12-20)

主引用文献

Buchwald, G.,Ebert, J.,Basquin, C.,Sauliere, J.,Jayachandran, U.,Bono, F.,Le Hir, H.,Conti, E.
Insights Into the Recruitment of the Nmd Machinery from the Crystal Structure of a Core Ejc-Upf3B Complex.
Proc.Natl.Acad.Sci.USA, 107:10050-, 2010

PubMed Abstract: In mammals, Up-frameshift proteins (UPFs) form a surveillance complex that interacts with the exon junction complex (EJC) to elicit nonsense-mediated mRNA decay (NMD). UPF3b is the component of the surveillance complex that bridges the interaction with the EJC. Here, we report the 3.4 A resolution crystal structure of a minimal UPF3b-EJC assembly, consisting of the interacting domains of five proteins (UPF3b, MAGO, Y14, eIF4AIII, and Barentsz) together with RNA and adenylyl-imidodiphosphate. Human UPF3b binds with the C-terminal domain stretched over a composite surface formed by eIF4AIII, MAGO, and Y14. Residues that affect NMD when mutated are found at the core interacting surfaces, whereas differences between UPF3b and UPF3a map at peripheral interacting residues. Comparison with the binding mode of the protein PYM underscores how a common molecular surface of MAGO and Y14 recognizes different proteins acting at different times in the same pathway. The binding mode to eIF4AIII identifies a surface hot spot that is used by different DEAD-box proteins to recruit their regulators.

PubMed: 20479275
DOI: 10.1073/PNAS.1000993107

実験手法

X-RAY DIFFRACTION (3.4 Å)