2XB0

DNA-binding domain from Saccharomyces cerevisiae chromatin- remodelling protein Chd1

2XB0 の概要

• エントリーDOI: 10.2210/pdb2xb0/pdb
• 分子名称: CHROMO DOMAIN-CONTAINING PROTEIN 1, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, dna-binding protein, transcription, chromatin regulator
• 由来する生物種: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)
• 細胞内の位置: Nucleus (Probable): P32657
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30569.76

構造登録者

Ryan, D.P.,Sundaramoorthy, R.,Owen-Hughes, T. (登録日: 2010-04-01, 公開日: 2011-05-11, 最終更新日: 2024-05-08)

主引用文献

Ryan, D.P.,Sundaramoorthy, R.,Martin, D.,Singh, V.,Owen-Hughes, T.
The DNA-Binding Domain of the Chd1 Chromatin- Remodelling Enzyme Contains Sant and Slide Domains.
Embo J., 30:2596-, 2011

PubMed Abstract: The ATP-dependent chromatin-remodelling enzyme Chd1 is a 168-kDa protein consisting of a double chromodomain, Snf2-related ATPase domain, and a C-terminal DNA-binding domain. Here, we show the DNA-binding domain is required for Saccharomyces cerevisiae Chd1 to bind and remodel nucleosomes. The crystal structure of this domain reveals the presence of structural homology to SANT and SLIDE domains previously identified in ISWI remodelling enzymes. The presence of these domains in ISWI and Chd1 chromatin-remodelling enzymes may provide a means of efficiently harnessing the action of the Snf2-related ATPase domain for the purpose of nucleosome spacing and provide an explanation for partial redundancy between these proteins. Site directed mutagenesis was used to identify residues important for DNA binding and generate a model describing the interaction of this domain with DNA. Through inclusion of Chd1 sequences in homology searches SLIDE domains were identified in CHD6-9 proteins. Point mutations to conserved amino acids within the human CHD7 SLIDE domain have been identified in patients with CHARGE syndrome.

PubMed: 21623345
DOI: 10.1038/EMBOJ.2011.166

実験手法

X-RAY DIFFRACTION (2 Å)