2X5V

80 microsecond laue diffraction snapshot from crystals of a photosynthetic reaction centre 3 millisecond following photoactivation.

2X5V の概要

• エントリーDOI: 10.2210/pdb2x5v/pdb
• 関連するPDBエントリー: 1DXR 1PRC 1R2C 1VRN 2JBL 2PRC 2WJM 2WJN 2X5U 3PRC 5PRC 6PRC 7PRC
• 分子名称: PHOTOSYNTHETIC REACTION CENTER CYTOCHROME C SUBUNIT, REACTION CENTER PROTEIN H CHAIN, REACTION CENTER PROTEIN L CHAIN, ... (10 entities in total)
• 機能のキーワード: lipidic-sponge phase, photosynthesis, electron transport, cell membrane, metal-binding, transmembrane, formylation, lipoprotein, lipids, transport
• 由来する生物種: BLASTOCHLORIS VIRIDIS; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 141263.14

構造登録者

Wohri, A.B.,Katona, G.,Johansson, L.C.,Fritz, E.,Malmerberg, E.,Andersson, M.,Vincent, J.,Eklund, M.,Cammarata, M.,Wulff, M.,Davidsson, J.,Groenhof, G.,Neutze, R. (登録日: 2010-02-10, 公開日: 2010-05-12, 最終更新日: 2024-11-06)

主引用文献

Wohri, A.B.,Katona, G.,Johansson, L.C.,Fritz, E.,Malmerberg, E.,Andersson, M.,Vincent, J.,Eklund, M.,Cammarata, M.,Wulff, M.,Davidsson, J.,Groenhof, G.,Neutze, R.
Light-Induced Structural Changes in a Photosynthetic Reaction Center Caught by Laue Diffraction.
Science, 328:630-, 2010

PubMed Abstract: Photosynthetic reaction centers convert the energy content of light into a transmembrane potential difference and so provide the major pathway for energy input into the biosphere. We applied time-resolved Laue diffraction to study light-induced conformational changes in the photosynthetic reaction center complex of Blastochloris viridis. The side chain of TyrL162, which lies adjacent to the special pair of bacteriochlorophyll molecules that are photooxidized in the primary light conversion event of photosynthesis, was observed to move 1.3 angstroms closer to the special pair after photoactivation. Free energy calculations suggest that this movement results from the deprotonation of this conserved tyrosine residue and provides a mechanism for stabilizing the primary charge separation reactions of photosynthesis.

PubMed: 20431017
DOI: 10.1126/SCIENCE.1186159

実験手法

X-RAY DIFFRACTION (3 Å)