2X34

Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown

2X34 の概要

• エントリーDOI: 10.2210/pdb2x34/pdb
• 関連するPDBエントリー: 2X32
• 分子名称: CELLULOSE-BINDING PROTEIN, X158, Ubiquinone-8 (3 entities in total)
• 機能のキーワード: carbohydrate-binding protein, oxidoreduction, marine bacteria, polyisoprenoid transport
• 由来する生物種: SACCHAROPHAGUS DEGRADANS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41003.77

構造登録者

Vincent, F.,Dal Molin, D.,Weiner, R.M.,Bourne, Y.,Henrissat, B. (登録日: 2010-01-19, 公開日: 2010-03-23, 最終更新日: 2024-10-23)

主引用文献

Vincent, F.,Dalmolin, D.,Weiner, R.M.,Bourne, Y.,Henrissat, B.
Structure of a Polyisoprenoid Binding Domain from Saccharophagus Degradans Implicated in Plant Cell Wall Breakdown
FEBS Lett., 584:1577-, 2010

PubMed Abstract: Saccharophagus degradans belongs to a recently discovered group of marine bacteria equipped with an arsenal of sugar cleaving enzymes coupled to carbohydrate-binding domains to degrade various insoluble complex polysaccharides. The modular Sde-1182 protein consists of a family 2 carbohydrate binding module linked to a X158 domain of unknown function. The 1.9 A and 1.55 A resolution crystal structures of the isolated X158 domain bound to the two related polyisoprenoid molecules, ubiquinone and octaprenyl pyrophosphate, unveil a beta-barrel architecture reminiscent of the YceI-like superfamily that resembles the architecture of the lipocalin fold. This unprecedented association coupling oxidoreduction and carbohydrate recognition events may have implications for effective nutrient uptake in the marine environment.

PubMed: 20227408
DOI: 10.1016/J.FEBSLET.2010.03.015

実験手法

X-RAY DIFFRACTION (1.9 Å)