2X1G

Crystal structure of Importin13 - Mago-Y14 complex

2X1G の概要

• エントリーDOI: 10.2210/pdb2x1g/pdb
• 関連するPDBエントリー: 1HL6 1OO0 1RK8
• 分子名称: RNA-BINDING PROTEIN 8A, PROTEIN MAGO NASHI, CADMUS (3 entities in total)
• 機能のキーワード: transport protein, developmental protein, mrna processing, nuclear transport, mrna splicing, mrna transport
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 295628.18

構造登録者

Bono, F.,Cook, A.G.,Gruenwald, M.,Ebert, J.,Conti, E. (登録日: 2009-12-23, 公開日: 2010-02-16, 最終更新日: 2023-12-20)

主引用文献

Bono, F.,Cook, A.G.,Grunwald, M.,Ebert, J.,Conti, E.
Nuclear Import Mechanism of the Ejc Component Mago- Y14 Revealed by Structural Studies of Importin 13.
Mol.Cell, 37:211-, 2010

PubMed Abstract: Mago and Y14 are core components of the exon junction complex (EJC), an assembly central to nonsense-mediated mRNA decay in humans and mRNA localization in flies. The Mago-Y14 heterodimer shuttles between the nucleus, where it is loaded onto specific mRNAs, and the cytoplasm, where it functions in translational regulation. The heterodimer is imported back into the nucleus by Importin 13 (Imp13), a member of the karyopherin-beta family of transport factors. We have elucidated the structural basis of the Mago-Y14 nuclear import cycle. The 3.35 A structure of the Drosophila Imp13-Mago-Y14 complex shows that Imp13 forms a ring-like molecule, reminiscent of Crm1, and encircles the Mago-Y14 cargo with a conserved interaction surface. The 2.8 A structure of human Imp13 bound to RanGTP reveals how Mago-Y14 is released in the nucleus by a steric hindrance mechanism. Comparison of the two structures suggests how this unusual karyopherin might function in bidirectional nucleocytoplasmic transport.

PubMed: 20122403
DOI: 10.1016/J.MOLCEL.2010.01.007

実験手法

X-RAY DIFFRACTION (3.35 Å)