2WZJ

Catalytic and UBA domain of kinase MARK2/(Par-1) K82R, T208E double mutant

Summary for 2WZJ

• Entry DOI: 10.2210/pdb2wzj/pdb
• Related: 1Y8G 1ZMU 1ZMV 1ZMW
• Descriptor: SERINE/THREONINE-PROTEIN KINASE MARK2 (2 entities in total)
• Functional Keywords: uba domain, transferase, serine/threonine-protein kinase, signaling protein, s/t protein kinase, differentiation, developmental protein
• Biological source: RATTUS NORVEGICUS (RAT)
• Total number of polymer chains: 6
• Total formula weight: 226220.72

Authors

Panneerselvam, S.,Marx, A.,Mandelkow, E.-M.,Mandelkow, E. (deposition date: 2009-11-30, release date: 2009-12-22, Last modification date: 2024-11-13)

Primary citation

Marx, A.,Nugoor, C.,Panneerselvam, S.,Mandelkow, E.
Structure and Function of Polarity-Inducing Kinase Family Mark/Par-1 within the Branch of Ampk/Snf1-Related Kinases.
Faseb J., 24:1637-, 2010

PubMed Abstract: Kinases of the MARK/Par-1 family of S/T protein kinases are regulators of diverse cellular processes in Caenorhabditis elegans, Drosophila, yeast, and mammalian cells. They are involved in nematode embryogenesis, epithelial cell polarization, cell signaling, and neuronal differentiation. MARK phosphorylates microtubule-associated proteins such as tau and is a key regulator of microtubule-based intracellular transport. Hyperphosphorylation of tau causes defects in neuronal transport and may induce abnormal aggregation of tau in Alzheimer disease and other tauopathies. Recent high-resolution structure analysis of MARK fragments covering the kinase domain and accessory regulatory domains has revealed important details regarding the autoregulation of MARK, but their interpretation has remained controversial. Here we focus on the structural aspects of MARK activity and autoregulation. Comparison of the available MARK structures with related kinases of the AMPK family and with new structures of MARK isoforms (MARK2 and 3) reveals unexpected structural similarities between these kinases that may help to resolve the existing controversies.

PubMed: 20071654
DOI: 10.1096/FJ.09-148064

Experimental method

X-RAY DIFFRACTION (2.786 Å)