2WW2

Structure of the Family GH92 Inverting Mannosidase BT2199 from Bacteroides thetaiotaomicron VPI-5482

2WW2 の概要

• エントリーDOI: 10.2210/pdb2ww2/pdb
• 関連するPDBエントリー: 2WVY
• 分子名称: ALPHA-1,2-MANNOSIDASE, (4S)-2-METHYL-2,4-PENTANEDIOL, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: hydrolase, glycoside hydrolase family 92, bt2199
• 由来する生物種: BACTEROIDES THETAIOTAOMICRON
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 252900.86

構造登録者

Suits, M.D.L.,Zhu, Y.,Thompson, A.,Gilbert, H.J.,Davies, G.J. (登録日: 2009-10-21, 公開日: 2009-12-29, 最終更新日: 2023-12-20)

主引用文献

Zhu, Y.,Suits, M.D.L.,Thompson, A.,Chavan, S.,Dinev, Z.,Dumon, C.,Smith, N.,Moremen, K.W.,Xiang, Y.,Siriwardena, A.,Williams, S.J.,Gilbert, H.J.,Davies, G.J.
Mechanistic Insights Into a Ca2+-Dependent Family of A-Mannosidases in a Human Gut Symbiont.
Nat.Chem.Biol., 6:125-, 2010

PubMed Abstract: Colonic bacteria, exemplified by Bacteroides thetaiotaomicron, play a key role in maintaining human health by harnessing large families of glycoside hydrolases (GHs) to exploit dietary polysaccharides and host glycans as nutrients. Such GH family expansion is exemplified by the 23 family GH92 glycosidases encoded by the B. thetaiotaomicron genome. Here we show that these are alpha-mannosidases that act via a single displacement mechanism to utilize host N-glycans. The three-dimensional structure of two GH92 mannosidases defines a family of two-domain proteins in which the catalytic center is located at the domain interface, providing acid (glutamate) and base (aspartate) assistance to hydrolysis in a Ca(2+)-dependent manner. The three-dimensional structures of the GH92s in complex with inhibitors provide insight into the specificity, mechanism and conformational itinerary of catalysis. Ca(2+) plays a key catalytic role in helping distort the mannoside away from its ground-state (4)C(1) chair conformation toward the transition state.

PubMed: 20081828
DOI: 10.1038/NCHEMBIO.278

実験手法

X-RAY DIFFRACTION (1.9 Å)