2WUY

the crystal structure of wild-type baculovirus polyhedra

2WUY の概要

• エントリーDOI: 10.2210/pdb2wuy/pdb
• 関連するPDBエントリー: 2WUX
• 分子名称: POLYHEDRIN (1 entity in total)
• 機能のキーワード: viral protein, microcrystals, polyhedra, virus, viral occlusion body, viral capsid
• 由来する生物種: AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28678.72

構造登録者

Ji, X.,Sutton, G.,Evans, G.,Axford, D.,Owen, R.,Stuart, D.I. (登録日: 2009-10-10, 公開日: 2009-12-15, 最終更新日: 2023-12-20)

主引用文献

Ji, X.,Sutton, G.,Evans, G.,Axford, D.,Owen, R.,Stuart, D.I.
How Baculovirus Polyhedra Fit Square Pegs Into Round Holes to Robustly Package Viruses.
Embo J., 29:505-, 2010

PubMed Abstract: Natural protein crystals (polyhedra) armour certain viruses, allowing them to survive for years under hostile conditions. We have determined the structure of polyhedra of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), revealing a highly symmetrical covalently cross-braced robust lattice, the subunits of which possess a flexible adaptor enabling this supra-molecular assembly to specifically entrap massive baculoviruses. Inter-subunit chemical switches modulate the controlled release of virus particles in the unusual high pH environment of the target insect's gut. Surprisingly, the polyhedrin subunits are more similar to picornavirus coat proteins than to the polyhedrin of cytoplasmic polyhedrosis virus (CPV). It is, therefore, remarkable that both AcMNPV and CPV polyhedra possess identical crystal lattices and crystal symmetry. This crystalline arrangement must be particularly well suited to the functional requirements of the polyhedra and has been either preserved or re-selected during evolution. The use of flexible adaptors to generate a powerful system for packaging irregular particles is characteristic of the AcMNPV polyhedrin and may provide a vehicle to sequester a wide range of objects such as biological nano-particles.

PubMed: 19959989
DOI: 10.1038/EMBOJ.2009.352

実験手法

X-RAY DIFFRACTION (3.09 Å)