2WUI

Crystal Structure of MexZ, a key repressor responsible for antibiotic resistance in Pseudomonas aeruginosa.

2WUI の概要

• エントリーDOI: 10.2210/pdb2wui/pdb
• 分子名称: TRANSCRIPTIONAL REGULATOR (1 entity in total)
• 機能のキーワード: gene regulation, transcription regulation, tetr, dna-binding, transcription
• 由来する生物種: PSEUDOMONAS AERUGINOSA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23761.29

構造登録者

Alguel, Y.,Lu, D.,Quade, N.,Zhang, X. (登録日: 2009-10-05, 公開日: 2010-08-18, 最終更新日: 2024-05-08)

主引用文献

Alguel, Y.,Lu, D.,Quade, N.,Sauter, S.,Zhang, X.
Crystal Structure of Mexz, a Key Repressor Responsible for Antibiotic Resistance in Pseudomonas Aeruginosa.
J.Struct.Biol., 172:305-, 2010

PubMed Abstract: Pseudomonas aeruginosa is responsible for around 10% of all hospital-acquired infections and the single most important pathogen of cystic fibrosis lungs. P. aeruginosa has high intrinsic and acquired antibiotic resistance, due to the extrusion of antibiotics by multidrug efflux pumps. The gene regulator MexZ controls the expression of mexXY, the efflux pump responsible for resistance to many drugs that are used for treating CF patients. MexZ is shown to be the most frequently mutated gene in P. aeruginosa isolated from CF patient lungs, confirming its importance in multidrug resistance. Here we present the crystal structure of MexZ at 2.9Å. Combining the structural information with biochemical data on key mutants identified, we provide an explanation for the structural and functional consequences of these mutants. This work provides a framework for further characterisation of MexZ in order to fully understand its regulation and induction.

PubMed: 20691272
DOI: 10.1016/J.JSB.2010.07.012

実験手法

X-RAY DIFFRACTION (2.9 Å)