2WSV

Galectin domain of porcine adenovirus type 4 NADC-1 isolate fibre complexed with lactose

2WSV の概要

• エントリーDOI: 10.2210/pdb2wsv/pdb
• 関連するPDBエントリー: 2WS0 2WST 2WSU 2WT1 2WT2
• 関連するBIRD辞書のPRD_ID: PRD_900008
• 分子名称: PUTATIVE FIBER PROTEIN, beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, NITRATE ION, ... (4 entities in total)
• 機能のキーワード: tandem-repeat-type, viral protein, carbohydrate recognition domain
• 由来する生物種: PORCINE ADENOVIRUS 4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38717.76

構造登録者

Guardado-Calvo, P.,Munoz, E.M.,Llamas-Saiz, A.L.,Fox, G.C.,Glasgow, J.N.,van Raaij, M.J. (登録日: 2009-09-10, 公開日: 2010-08-11, 最終更新日: 2023-12-20)

主引用文献

Guardado-Calvo, P.,Munoz, E.M.,Llamas-Saiz, A.L.,Fox, G.C.,Kahn, R.,Curiel, D.T.,Glasgow, J.N.,van Raaij, M.J.
Crystallographic structure of porcine adenovirus type 4 fiber head and galectin domains.
J. Virol., 84:10558-10568, 2010

PubMed Abstract: Adenovirus isolate NADC-1, a strain of porcine adenovirus type 4, has a fiber containing an N-terminal virus attachment region, shaft and head domains, and a C-terminal galectin domain connected to the head by an RGD-containing sequence. The crystal structure of the head domain is similar to previously solved adenovirus fiber head domains, but specific residues for binding the coxsackievirus and adenovirus receptor (CAR), CD46, or sialic acid are not conserved. The structure of the galectin domain reveals an interaction interface between its two carbohydrate recognition domains, locating both sugar binding sites face to face. Sequence evidence suggests other tandem-repeat galectins have the same arrangement. We show that the galectin domain binds carbohydrates containing lactose and N-acetyl-lactosamine units, and we present structures of the galectin domain with lactose, N-acetyl-lactosamine, 3-aminopropyl-lacto-N-neotetraose, and 2-aminoethyl-tri(N-acetyl-lactosamine), confirming the domain as a bona fide galectin domain.

PubMed: 20686025
DOI: 10.1128/JVI.00997-10

実験手法

X-RAY DIFFRACTION (2 Å)