2WSP

Thermotoga maritima alpha-L-fucosynthase, TmD224G, in complex with alpha-L-Fuc-(1-2)-beta-L-Fuc-N3

2WSP の概要

• エントリーDOI: 10.2210/pdb2wsp/pdb
• 関連するPDBエントリー: 1HL8 1HL9 1ODU
• 分子名称: ALPHA-L-FUCOSIDASE, PUTATIVE, alpha-L-fucopyranose-(1-2)-beta-L-fucosyl-azide (3 entities in total)
• 機能のキーワード: hydrolase, glycoside hydrolase, carbohydrate synthesis, thermophilic enzyme
• 由来する生物種: THERMOTOGA MARITIMA
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 105100.66

構造登録者

Sulzenbacher, G.,Lipski, A.,Cobucci-Ponzano, B.,Conte, F.,Bedini, E.,Corsaro, M.M.,Parrilli, M.,Dal Piaz, F.,Lepore, L.,Rossi, M.,Moracci, M. (登録日: 2009-09-08, 公開日: 2010-01-19, 最終更新日: 2024-10-09)

主引用文献

Cobucci-Ponzano, B.,Conte, F.,Bedini, E.,Corsaro, M.M.,Parrilli, M.,Sulzenbacher, G.,Lipski, A.,Dal Piaz, F.,Lepore, L.,Rossi, M.,Moracci, M.
Beta-Glycosyl Azides as Substrates for Alpha-Glycosynthases: Preparation of Novel Efficient Alpha-L-Fucosynthases
Chem.Biol., 16:1097-, 2009

PubMed Abstract: Fucose-containing oligosaccharides play a central role in physio-pathological events, and fucosylated oligosaccharides have interesting potential applications in biomedicine. No methods for the large-scale production of oligosaccharides are currently available, but the chemo-enzymatic approach is very promising. Glycosynthases, mutated glycosidases that synthesize oligosaccharides in high yields, have been demonstrated to be an interesting alternative. However, examples of glycosynthases available so far are restricted to a limited number of glycosidases families and to only one retaining alpha-glycosynthase. We show here that new mutants of two alpha-L-fucosidases are efficient alpha-L-fucosynthases. The approach shown utilized beta-L-fucopyranosyl azide as donor substrate leading to transglycosylation yields up to 91%. This is the first method exploiting a beta-glycosyl azide donor for alpha-glycosynthases; its applicability to the glycosynthetic methodology in a wider perspective is presented.

PubMed: 19875083
DOI: 10.1016/J.CHEMBIOL.2009.09.013

実験手法

X-RAY DIFFRACTION (2.65 Å)