2WQP

Crystal structure of sialic acid synthase NeuB-inhibitor complex

2WQP の概要

• エントリーDOI: 10.2210/pdb2wqp/pdb
• 分子名称: POLYSIALIC ACID CAPSULE BIOSYNTHESIS PROTEIN SIAC, 5-(ACETYLAMINO)-3,5-DIDEOXY-2-O-PHOSPHONO-D-ERYTHRO-L-MANNO-NONONIC ACID, MANGANESE (II) ION, ... (7 entities in total)
• 機能のキーワード: neub, inhibitor, tim barrel, sialic acid synthase, transferase
• 由来する生物種: NEISSERIA MENINGITIDIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39768.33

構造登録者

Liu, F.,Lee, H.J.,Strynadka, N.C.J.,Tanner, M.E. (登録日: 2009-08-25, 公開日: 2009-09-15, 最終更新日: 2024-11-13)

主引用文献

Liu, F.,Lee, H.J.,Strynadka, N.C.J.,Tanner, M.E.
The Inhibition of Neisseria Meningitidis Sialic Acid Synthase by a Tetrahedral Intermediate Analog.
Biochemistry, 48:9194-, 2009

PubMed Abstract: The Neisseria meningitidis sialic acid synthase (NeuB) catalyzes the metal-dependent condensation of N-acetylmannosamine (ManNAc) and phosphoenolpyruvate (PEP) to generate N-acetylneuraminic acid (NeuAc or sialic acid). N. meningitidis is a causative agent of meningitis and produces a capsular polysaccharide comprised of polysialic acid. This allows it to evade the immune system of the host by an act of molecular mimicry. This work describes the synthesis and characterization of the first potent inhibitor of sialic acid synthase. The inhibitor is a stable deoxy analogue of the tetrahedral intermediate presumed to form in the NeuB reaction and was synthesized as a mixture of stereoisomers at the key tetrahedral center. Inhibition studies demonstrate that one stereoisomer binds more tightly than the other and that the more potent isomer binds with micromolar affinity. An X-ray crystallographic analysis of the NeuB.inhibitor.Mn(2+) complex solved to a resolution of 1.75 A shows that the more tightly bound stereoisomer bears a (2R)-configuration. This suggests that the tetrahedral intermediate formed in the NeuB reaction also bears a (2R)-configuration. This analysis is consistent with a mechanism whereby the active site metal plays at least two roles during catalysis. First, it serves as an electrostatic catalyst and activates the aldehyde of ManNAc for attack by the alkene of PEP. Second, it serves as a source of nucleophilic water and delivers it to the si face of the oxocarbenium intermediate to generate a tetrahedral intermediate with a (2R)-configuration.

PubMed: 19719325
DOI: 10.1021/BI9012758

実験手法

X-RAY DIFFRACTION (1.75 Å)