2WPV

Crystal structure of S. cerevisiae Get4-Get5 complex

Summary for 2WPV

• Entry DOI: 10.2210/pdb2wpv/pdb
• Descriptor: UPF0363 PROTEIN YOR164C, UBIQUITIN-LIKE PROTEIN MDY2, MERCURY (II) ION, ... (4 entities in total)
• Functional Keywords: golgi-er trafficking, tail-anchored protein, protein binding, get5, get4
• Biological source: SACCHAROMYCES CEREVISIAE (BAKER'S YEAST); More
• Cellular location: Cytoplasm: Q12125; Cytoplasm, cytosol: Q12285
• Total number of polymer chains: 8
• Total formula weight: 172622.01

Authors

Chang, Y.-W.,Chuang, Y.-C.,Ho, Y.-C.,Cheng, M.-Y.,Sun, Y.-J.,Hsiao, C.-D.,Wang, C. (deposition date: 2009-08-11, release date: 2010-01-26, Last modification date: 2024-05-08)

Primary citation

Chang, Y.-W.,Chuang, Y.-C.,Ho, Y.-C.,Cheng, M.-Y.,Sun, Y.-J.,Hsiao, C.-D.,Wang, C.
Crystal Structure of Get4-Get5 Complex and its Interactions with Sgt2, Get3, and Ydj1.
J.Biol.Chem., 285:9962-, 2010

PubMed Abstract: Get3, Get4, and Get5 in Saccharomyces cerevisiae participate in the insertion of tail-anchored proteins into the endoplasmic reticulum membrane. We elucidated the interaction between Get4 and Get5 and investigated their interaction with Get3 and a tetratricopeptide repeat-containing protein, Sgt2. Based on co-immunoprecipitation and crystallographic studies, Get4 and Get5 formed a tight complex, suggesting that they constitute subunits of a larger complex. In contrast, although Get3 interacted physically with the Get4-Get5 complex, low amounts of Get3 co-precipitated with Get5, implying a transient interaction between Get3 and Get4-Get5. Sgt2 also interacted with Get5, although the amount of Sgt2 that co-precipitated with Get5 varied. Moreover, GET3, GET4, and GET5 interacted genetically with molecular chaperone YDJ1, suggesting that chaperones might also be involved in the insertion of tail-anchored proteins.

PubMed: 20106980
DOI: 10.1074/JBC.M109.087098

Experimental method

X-RAY DIFFRACTION (1.99 Å)