2WNB

Crystal Structure of a Mammalian Sialyltransferase in complex with disaccharide and CMP

2WNB の概要

• エントリーDOI: 10.2210/pdb2wnb/pdb
• 関連するPDBエントリー: 2WML 2WNF
• 関連するBIRD辞書のPRD_ID: PRD_900084
• 分子名称: CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1, beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-alpha-D-galactopyranose, CYTIDINE-5'-MONOPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: glycosyltransferase, alternative splicing, disulfide bond, golgi apparatus, sialyltransferase, glycoprotein, signal-anchor, transmembrane, membrane, secreted, transferase, sialic acid
• 由来する生物種: Sus scrofa (pig)
• 細胞内の位置: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein: Q02745
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35594.61

構造登録者

Rao, F.V.,Rich, J.R.,Raikic, B.,Wakarchuk, W.W.,Withers, S.G.,Strynadka, N.C.J. (登録日: 2009-07-08, 公開日: 2009-10-13, 最終更新日: 2024-10-09)

主引用文献

Rao, F.V.,Rich, J.R.,Rakic, B.,Buddai, S.,Schwartz, M.F.,Johnson, K.,Bowe, C.,Wakarchuk, W.W.,Defrees, S.,Withers, S.G.,Strynadka, N.C.J.
Structural Insight Into Mammalian Sialyltransferases.
Nat.Struct.Mol.Biol., 16:1186-, 2009

PubMed Abstract: Mammalian cell surfaces are modified by complex arrays of glycoproteins, glycolipids and polysaccharides, many of which terminate in sialic acid and have central roles in essential processes including cell recognition, adhesion and immunogenicity. Sialylation of glycoconjugates is performed by a set of sequence-related enzymes known as sialyltransferases (STs). Here we present the crystal structure of a mammalian ST, porcine ST3Gal-I, providing a structural basis for understanding the mechanism and specificity of these enzymes and for the design of selective inhibitors.

PubMed: 19820709
DOI: 10.1038/NSMB.1685

実験手法

X-RAY DIFFRACTION (1.55 Å)