2WBE

Kinesin-5-Tubulin Complex with AMPPNP

2WBE の概要

• エントリーDOI: 10.2210/pdb2wbe/pdb
• EMDBエントリー: 1604
• 分子名称: TUBULIN ALPHA-1D CHAIN, TUBULIN BETA-2B CHAIN, BIPOLAR KINESIN KRP-130, ... (8 entities in total)
• 機能のキーワード: eg5, klp61f, kinesin, tubulin, mitosis, kinesin-5, gtp-binding, motor protein, cell division, cell cycle, microtubule, atp-binding, homology model, phosphoprotein, nucleotide-binding, structural protein
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 144301.67

構造登録者

Bodey, A.J.,Kikkawa, M.,Moores, C.A. (登録日: 2009-02-26, 公開日: 2009-03-24, 最終更新日: 2024-05-08)

主引用文献

Bodey, A.J.,Kikkawa, M.,Moores, C.A.
9-Angstrom Structure of a Microtubule-Bound Mitotic Motor.
J.Mol.Biol., 388:218-, 2009

PubMed Abstract: Kinesin-5 (K5) motors are important components of the microtubule (MT)-based cell division machinery and are targets for small-molecule inhibitors currently in cancer clinical trials. However, the nature of the K5-MT interaction and the regulatory mechanisms that control it remain unclear. Using cryo-electron microscopy and image processing, we calculated the structure of a K5 motor bound to MTs at 9 A resolution, providing insight into this important interaction. Our reconstruction reveals the K5 motor domain in an ATP-like conformation in which MT binding induces the conserved nucleotide-sensing switch I and II loops to form a compact subdomain around the bound nucleotide. Our reconstruction also reveals a novel conformation for the K5-specific drug-binding loop 5, suggesting a possible role for it in switching K5s between force generation and diffusional modes of MT binding. Our data thus shed light on regulation of the interaction between spindle components important for chromosome segregation.

PubMed: 19285086
DOI: 10.1016/J.JMB.2009.03.008

実験手法

ELECTRON MICROSCOPY (9.4 Å)