2W8C

Plastocyanin variant with N-terminal Methionine - closed structure

Summary for 2W8C

• Entry DOI: 10.2210/pdb2w8c/pdb
• Related: 1BAW 2W88
• Descriptor: PLASTOCYANIN, COPPER (II) ION, ZINC ION, ... (4 entities in total)
• Functional Keywords: protein interactions, transport, cupredoxin, self-assembly, metal-binding, electron transport
• Biological source: PHORMIDIUM LAMINOSUM
• Total number of polymer chains: 2
• Total formula weight: 23514.72

Authors

Crowley, P.B.,Matias, P.M.,Khan, A.R. (deposition date: 2009-01-15, release date: 2009-10-27, Last modification date: 2023-12-13)

Primary citation

Crowley, P.B.,Matias, P.M.,Khan, A.R.,Roessle, M.,Svergun, D.I.
Metal-Mediated Self-Assembly of a Beta-Sandwich Protein.
Chemistry, 15:12672-, 2009

PubMed Abstract: The beta-sandwich cupredoxin Plastocyanin (Pc) was found to self-assemble in the presence of Zn(2+), a known mediator of protein-protein interfaces. Diffraction-quality crystals of Pc grew from solutions containing zinc acetate as the sole precipitant. Di- and trinuclear zinc sites contribute to the crystal contacts in this structure. A different crystal form, also involving numerous zinc bridging ions, was obtained in the presence of poly(ethylene glycol) 8 000. Comparison of the two crystal forms reveals the effect of macromolecular crowding on self-assembly. Solution-state structural characterisation of the Zn(2+)-mediated Pc oligomers was performed by using a combination of chemical shift perturbation mapping and small-angle X-ray scattering. The data indicate the formation of dimers in solution. The implications for metal-mediated assembly and crystallisation are discussed.

PubMed: 19834935
DOI: 10.1002/CHEM.200901410

Experimental method

X-RAY DIFFRACTION (1.8 Å)