2W7N

Crystal Structure of KorA Bound to Operator DNA: Insight into Repressor Cooperation in RP4 Gene Regulation

2W7N の概要

• エントリーDOI: 10.2210/pdb2w7n/pdb
• 分子名称: TRFB TRANSCRIPTIONAL REPRESSOR PROTEIN, 5'-D(*AP*AP*TP*GP*TP*TP*TP*AP*GP*CP *TP*AP*AP*AP*CP*AP*AP*G)-3', 5'-D(*CP*BRUP*BRUP*GP*TP*TP*TP*AP*GP*CP*TP*AP *AP*AP*CP*AP*BRUP*T)-3', ... (4 entities in total)
• 機能のキーワード: incp, plasmid, repressor, dna-binding, transcription/dna, protein-dna complex, transcription factor, helix-turn-helix motif, transcription regulation, transcription-dna complex
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 45093.47

構造登録者

Koenig, B.,Mueller, J.J.,Lanka, E.,Heinemann, U. (登録日: 2008-12-23, 公開日: 2009-02-17, 最終更新日: 2024-05-08)

主引用文献

Koenig, B.,Mueller, J.J.,Lanka, E.,Heinemann, U.
Crystal Structure of Kora Bound to Operator DNA: Insight Into Repressor Cooperation in Rp4 Gene Regulation
Nucleic Acids Res., 37:1915-, 2009

PubMed Abstract: KorA is a global repressor in RP4 which regulates cooperatively the expression of plasmid genes whose products are involved in replication, conjugative transfer and stable inheritance. The structure of KorA bound to an 18-bp DNA duplex that contains the symmetric operator sequence and incorporates 5-bromo-deoxyuridine nucleosides has been determined by multiple-wavelength anomalous diffraction phasing at 1.96-A resolution. KorA is present as a symmetric dimer and contacts DNA via a helix-turn-helix motif. Each half-site of the symmetric operator DNA binds one copy of the protein in the major groove. As confirmed by mutagenesis, recognition specificity is based on two KorA side chains forming hydrogen bonds to four bases within each operator half-site. KorA has a unique dimerization module shared by the RP4 proteins TrbA and KlcB. We propose that these proteins cooperate with the global RP4 repressor KorB in a similar manner via this dimerization module and thus regulate RP4 inheritance.

PubMed: 19190096
DOI: 10.1093/NAR/GKP044

実験手法

X-RAY DIFFRACTION (1.85 Å)