2W4T

ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE

Summary for 2W4T

• Entry DOI: 10.2210/pdb2w4t/pdb
• Related: 1B7T 1DFK 1DFL 1KK7 1KK8 1KQM 1KWO 1L2O 1QVI 1S5G 1SCM 1SR6 1WDC 2W4V 2W4W
• EMDB information: 1561
• Descriptor: MYOSIN HEAVY CHAIN, STRIATED MUSCLE, MYOSIN REGULATORY LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE, MYOSIN ESSENTIAL LIGHT CHAIN, STRIATED ADDUCTOR MUSCLE (3 entities in total)
• Functional Keywords: contractile protein, nucleotide-binding, tropomyosin, light chains, actin- binding, freeze substitution, isometric contraction, freezing, microtomy, atp-binding, thin filament, motor protein, thick filament, muscle protein, image processing, calmodulin-binding, actin, insect, myosin, muscle, troponin, multivariate data analysis
• Biological source: ARGOPECTEN IRRADIANS (BAY SCALLOP); More
• Total number of polymer chains: 3
• Total formula weight: 127471.49

Authors

Wu, S.,Liu, J.,Reedy, M.C.,Tregear, R.T.,Winkler, H.,Franzini-Armstrong, C.,Sasaki, H.,Lucaveche, C.,Goldman, Y.E.,Reedy, M.K.,Taylor, K.A. (deposition date: 2008-12-02, release date: 2010-01-19, Last modification date: 2024-05-08)

Primary citation

Wu, S.,Liu, J.,Reedy, M.C.,Tregear, R.T.,Winkler, H.,Franzini-Armstrong, C.,Sasaki, H.,Lucaveche, C.,Goldman, Y.E.,Reedy, M.K.,Taylor, K.A.
Electron Tomography of Cryofixed, Isometrically Contracting Insect Flight Muscle Reveals Novel Actin-Myosin Interactions
Plos One, 5:12643-, 2010

PubMed Abstract: Isometric muscle contraction, where force is generated without muscle shortening, is a molecular traffic jam in which the number of actin-attached motors is maximized and all states of motor action are trapped with consequently high heterogeneity. This heterogeneity is a major limitation to deciphering myosin conformational changes in situ.

PubMed: 20844746
DOI: 10.1371/JOURNAL.PONE.0012643

Experimental method

ELECTRON MICROSCOPY (35 Å)