2W4G

ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE QUICK FROZEN AFTER A QUICK STRETCH STEP

Summary for 2W4G

• Entry DOI: 10.2210/pdb2w4g/pdb
• Related: 1ALM 1I84 1M8Q 1MVW 1O18 1O19 1O1A 1O1B 1O1C 1O1D 1O1E 1O1F 1O1G 2MYS 2W4A
• EMDB information: 1584 1585
• Descriptor: MYOSIN REGULATORY LIGHT CHAIN 2, SKELETAL MUSCLE ISOFORM, MYOSIN LIGHT CHAIN 3, SKELETAL MUSCLE ISOFORM, MYOSIN HEAVY CHAIN, SKELETAL MUSCLE, ADULT (3 entities in total)
• Functional Keywords: contractile protein, methylation, atp-binding, isometric contraction, microtomy, freeze substitution, muscle protein, calmodulin-binding, motor protein, actin-binding
• Biological source: GALLUS GALLUS (CHICKEN); More
• Total number of polymer chains: 3
• Total formula weight: 129033.46

Authors

Wu, S.,Liu, J.,Reedy, M.C.,Tregear, R.T.,Winkler, H.,Franzini-Armstrong, C.,Sasaki, H.,Lucaveche, C.,Goldman, Y.E.,Reedy, M.K.,Taylor, K.A. (deposition date: 2008-11-25, release date: 2010-08-25, Last modification date: 2024-05-08)

Primary citation

Wu, S.,Liu, J.,Reedy, M.C.,Tregear, R.T.,Winkler, H.,Franzini-Armstrong, C.,Sasaki, H.,Lucaveche, C.,Goldman, Y.E.,Reedy, M.K.,Taylor, K.A.
Electron Tomography of Cryofixed, Isometrically Contracting Insect Flight Muscle Reveals Novel Actin-Myosin Interactions
Plos One, 5:12643-, 2010

PubMed Abstract: Isometric muscle contraction, where force is generated without muscle shortening, is a molecular traffic jam in which the number of actin-attached motors is maximized and all states of motor action are trapped with consequently high heterogeneity. This heterogeneity is a major limitation to deciphering myosin conformational changes in situ.

PubMed: 20844746
DOI: 10.1371/JOURNAL.PONE.0012643

Experimental method

ELECTRON MICROSCOPY (35 Å)